Fig. 4

Dynamic cellular and transcriptomic changes in leukemic cells after chemotherapy in P116, P105, and P115. a,c UMAP plots of leukemic cells from pre- and post-therapy samples for each patient, with cells color-coded by sample origin (a) and cell type (c). b Bar plot showing the number of leukemic cells in samples described in a. d Bar plot depicting the distribution of cell types in samples described in c. e Violin plots of normalized single-cell expression scores for LSC and OXPHOS signatures in HSC-like and LMPP-like cells from patients P116 and P105, with black dots representing average signature expression. P values were calculated using the Wilcoxon signed-rank test. f Heatmap visualization of Metascape pathways enriched by upregulated genes in each cell population from post-therapy samples compared to pre-therapy samples. g Violin plots showing the expression of genes related to heme metabolism signaling pathways and response to reactive oxygen species in pre- and post-therapy cell populations