Fig. 2

Differentiation trajectory of phloem cells. A A successive differentiation trajectory from phloem mother cells to SEs and CCs. Each dot indicates a single cell. The black arrow indicates the start of the trajectory. B Expression patterns of phloem-specific genes (AUX1, PIN1, APL, FTIP1, SEOR1, and CalS7). The colors represent expression levels of these genes in individual cells. C RNA in situ hybridization of APL with the sense probe as a negative control. Scale bars, 100 μm. D Heatmap showing expression of the branch-dependent genes over pseudo-time. Representative marker genes are shown on the right of the heatmap. Both sides of the heatmap are the end of pseudo-time. E Dot plot of GO enrichment analysis of the identified cell clusters. ER, endoplasmic reticulum. L-Glu, L-glutamate