Fig. 3
From: Linking single-cell measurements of mass, growth rate, and gene expression
Linked biophysical and gene expression measurements of activated murine CD8+ T cells. a Plot of mass accumulation rate versus buoyant mass for murine CD8+ T cells after 24 h (green points, n = 59) or 48 h (blue triangles, n = 49) of activation in vitro. Kernel density plots, using the same color scheme, are included on the margins for both populations. ***P < 0.001, N.S. indicates not significant; Mann-Whitney U test. b Plot of mass-normalized single-cell growth rates (growth efficiency) for the same murine CD8+ T cells activated for 24 or 48 h in vitro. Groups were compared with a Mann-Whitney U test (***P < 0.001). c Box charts showing the Spearman correlation coefficients between single-cell mass measurements and the expression of a subset of genes previously found to be related to cell cycle in activated CD8+ T cells (300 genes) for cells activated for 24 or 48 h. For comparison, the null distribution of Spearman correlation coefficients for the same subset of cells after randomly assigning single-cell mass measurements is shown for each time point (gray boxes, Methods). Groups were compared with a Mann-Whitney U test (***P < 0.001, **P < 0.01)