Fig. 9

Off-target analysis in cells and animals with chromosome elimination. a Summary of indels detected by WGS. See Additional file 1: Table S3 for corresponding mice and cell lines. b Summary of genomic rearrangements on the target sequences detected by WGS. c Circos plots showing identified off-target hotspots. Lines link bait site (Kdm5d) to identified off-target hotspots of indicated CRISPR/Cas9 with a range of color from white to dark red. The higher the frequency, the darker the color. The number of identified off-targets for each CRISPR/Cas9 is shown in the middle of the circus plot. Arrowheads indicate the bait CRISPR/Cas9 Kdm5d site, but note that the bait CRISPR/Cas9 Kdm5d has no detected off-target sites. Three repeats for each treatment (Additional file 1: Table S6 and S7)