Fig. 4
From: CRISPR/Cas9-mediated genome editing induces exon skipping by alternative splicing or exon deletion
An sgRNA targeting exon 23 of Dmd can partially restore in-frame dystrophin mRNA. a Schematic of sgRNA targeting and skipping of mouse Dmd exon 23 and location of primers for RT-PCR analysis. Skipping of exon 23 will generate in-frame mRNA. b sgRNA target sites in Dmd exon 23. c RT-PCR analysis of C2C12 mouse myoblast cells transduced with lentiviruses that encode Cas9 and sgDmd1, 2, 3, or 4. The expected band sizes are 353 bp and 140 bp. M molecular marker. d Sequence analysis of the 140-bp cDNA band from sgDmd2-treated cells confirmed splicing of exon 22 to exon 24