Mouse genomic screen reveals novel host regulator of metastasis
© The Author(s). 2017
Published: 16 February 2017
Tumor cells have to overcome challenges in the host tissue microenvironment to metastasize successfully to distant organs. In a recent Nature study, a genome-wide functional screen demonstrated that deficiency of the sphingosine-1-phoshate (S1P) transporter gene Spns2 in endothelium increased immune-mediated cell killing by T cells and natural killer (NK) cells, thereby suppressing metastatic colonization.
Metastasis is a highly inefficient process—less than 0.02% of disseminated tumor cells (DTCs) are believed to be capable of seeding secondary tumors. Much of the high rate of attrition of DTCs occurs during the colonization step, where the arriving DTCs face a challenging microenvironment that is often distinctively different from that of the primary tumor . Application of transcriptomic profiling and genome-wide functional screening strategies based on RNA interference, CRISPR-Cas9 genome editing, or transposon mutagenesis technologies have led to the discovery of various tumor-cell-intrinsic factors that are important for successful metastatic colonization in various target organs . Pioneering work by Hunter and others using mouse genetic crossing has also revealed the critical role of the host genetic background in determining metastatic efficiency . However, genetic screening of host tissue regulators of metastasis has been difficult and rarely attempted previously. In a recent issue of Nature, van der Weyden and colleagues used 810 mutant mouse strains to conduct a tour de force in vivo screen of host genes involved in regulating metastatic colonization . The study revealed that deficiency of Spns2, which encodes a transporter of sphingosine-1-phoshate (S1P) that regulates lymphocyte trafficking, strongly suppressed lung metastasis colonization by increasing the effector-T-cell- and natural killer (NK)-cell-mediated immune defense present at distant organs.
A major hurdle for successful metastatic colonization of DTCs is the host tissue immune system . It has been shown that cytotoxic T cells are critical for preventing metastatic colonization of melanoma in the lungs , and that NK cell depletion increases metastasis . Metastatic cancer cells have been reported to develop mechanisms to suppress the host immune system  or evade immune cell killing . Interestingly, previous studies also indicate that host tissue polymorphisms can alter the risk of cancer metastasis by affecting immune surveillance of cancer. For example, Cadm1 is a metastasis susceptibility locus gene that suppresses metastasis by sensitizing tumor cells to T-cell-mediated killing .
In the Nature study, the authors tested lung metastasis efficiency of the B16-F10 metastatic mouse melanoma cell line in 810 randomly selected mutant mouse strains that are defective in genes involved in a wide range of biological functions. They identified 23 host mutations that significantly decreased or increased the number of lung metastatic lesions. Interestingly, 19 out of these 23 mutant mouse strains displayed immune-related phenotypes, which implied a prominent involvement of the host immune system in regulating metastatic colonization. Not surprisingly, mutations that caused deficiency in the interferon response, such as loss of the interferon regulatory factor genes Irf1 and Irf7, resulted in an increased incidence of metastasis. On the other hand, mutations in 15 genes, including many that have not previously been implicated in metastasis, reduced the rate of metastasis. The strongest metastatic suppression was observed in the Spns2 tm1a/tm1a mutant mouse strain. While primary tumor growth was not affected in Spns2 mutant mice, spontaneous and experimental metastasis to lung and liver was reduced when they were injected with metastatic melanoma, colorectal, or breast cancer cell lines. Importantly, although Spns2 mutation did not affect the initial dissemination and extravasation of cancer cells, an increased number of apoptotic cancer cells were observed in the lung. These findings indicate that host SPNS2 fosters a more favorable environment for the survival of DTCs in the lung.
SPNS2 is a cell surface protein that transports intracellular S1P to blood and lymph, where S1P acts as a bioactive lipid mediator that binds to its G-protein-coupled receptor to regulate cell survival, proliferation, migration, angiogenesis, lymphangiogenesis, lymphocyte trafficking, and immune response . Consistent with the critical role of SPNS2 in S1P transport, Spns2 tm1a/tm1a mice have lower levels of S1P in serum and increased levels in the lung, which resulted in a profound alteration of leukocyte trafficking in the animals. In addition to a significant reduction of T and B cells in circulation, a dramatic increase in the NK-cell population and a reduced T-cell percentage were observed in the lungs of Spns2 tm1a/tm1a mice.
At first glance, it seems counterintuitive that a decreased lymphocyte count weakens metastatic colonization. However, the authors went on to demonstrate that despite a general decrease in T cells, the ratio of effector T cells and immunosuppressive regulatory T (Treg) cells was increased in the lungs of Spns2-deficient mice, as were NK cell numbers. In addition, CD4+ and CD8+ cells from Spns2 tm1a/tm1a animals showed a stronger degranulation response, increased interferon-γ production, and more effective B16-F10 tumor cell killing in vitro, indicating higher T-cell activity. In vivo T-cell and NK-cell depletion experiments showed that combined depletion of CD8+ T cells and NK cells, but not either population alone, restored the metastatic efficiency of cancer cells in Spns2 tm1a/tm1a mice to the levels observed in wild-type mice. Similar findings were observed in the liver, demonstrating that both T cells and NK cells are responsible for and work cooperatively to provide defense against metastasis in different organs. Finally, treatment of wild-type mice with 4′-deoxypyridoxine (DOP), which inhibits S1P degradation and thus increases S1P levels, led to a similar increase in immune-mediated killing and suppression of lung metastasis as observed in the Spns2 tm1a/tm1a mice. This result indicates that S1P levels regulate lymphocyte circulation and modulate the percentage of effector T cells and NK cells in the lung, thereby offering an attractive potential therapeutic target for metastatic colonization (Fig. 1).
While previous research in the field had revealed intricate networks of tumor–stromal interactions during metastasis, the current study represents the first extensive in vivo screen of host factors that influence metastatic colonization. Several other genes identified in the screen still remained uncharacterized for their functional mechanism in metastasis, representing many additional avenues for future explorations. Further efforts on similar in vivo functional screens should focus on “druggable” classes of genes with no life-threatening phenotypes in mutant mice, as these genes are likely ideal targets for therapeutic intervention.
Consistent with the findings observed in mouse models, SPNS2 has been reported to be upregulated in the stromal gene expression signature associated with a poor clinical outcome of human breast cancer , which underscores the clinical relevance of the present study. The identification of SPNS2 as a novel regulator of the host immune response to metastasis further highlights the essential role of resident immune cells in guarding and protecting against metastasis. The relevance of this mechanism is particularly salient, as immunotherapy has been proven to have striking effects in metastatic cancer patients, especially in those with metastatic melanoma . It is unclear why SPNS2 deficiency specifically affects the development of metastases but has no impact on primary tumor growth. It is possible that initial metastatic seeding is particularly sensitive to immune clearance, while established tumors are more refractory to the attack of effector T cells and NK cells. Alternatively, the immune microenvironment in the lung and other organs may be more responsive to S1P levels. Future studies need to investigate these questions, and explore the therapeutic effect of S1P and SPNS2 inhibition in established metastasis, which more closely mimics the clinical situation of late-stage cancer patients.
There are several potential translational applications of these new insights into SPNS2 and S1P as functional regulators of metastasis. As circulating S1P levels are tightly controlled by SPNS2, and increasing S1P levels with DOP treatment can suppress metastasis, S1P could be used as a biomarker of metastasis susceptibility in cancer patients. Such analysis should be performed after stratifying patients based on their cancer subtypes, as varying degrees of immune infiltration have been reported in different subtypes of breast cancer and other cancers. Targeting S1P using blocking antibodies such as sphingomab could be complicated by potential side effects in the immune and vascular systems. Alternatively, because it is a cell surface transporter, neutralizing antibodies or small inhibitors against SPNS2 could be developed as agents to prevent or reduce metastasis. In addition, genetic polymorphism, somatic mutations, or other means of increased expression or activity of SPNS2 and other components of the S1P pathway may play a causal role in promoting cancer metastasis, which should be an important topic for future investigations. Overall, this study represents a novel approach to study the complicated role of host tissue in cancer metastasis and opens up a potential new avenue to increase the efficacy of immunotherapy for metastatic cancer.
Disseminated tumor cells
- Treg :
Regulatory T cell
The authors’ laboratory is supported by a Susan G. Komen Fellowship to Toni Celià-Terrassa (PDF15332075), and grants from the Brewster Foundation, the Breast Cancer Research Foundation, Department of Defense (BC123187), and the National Institutes of Health (R01CA141062) to Yibin Kang.
TCT and YK wrote the article and prepared the associated figure. Both authors read and approved the final manuscript.
The authors declare that they have no competing interests.
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