Erratum to: Quartz-Seq: a highly reproducible and sensitive single-cell RNA sequencing method, reveals non-genetic gene-expression heterogeneity
- Yohei Sasagawa†1, 7,
- Itoshi Nikaido†1, 7,
- Tetsutaro Hayashi2,
- Hiroki Danno3,
- Kenichiro D Uno1,
- Takeshi Imai4, 5 and
- Hiroki R. Ueda1, 3, 6Email author
© The Author(s). 2017
Received: 12 January 2017
Accepted: 12 January 2017
Published: 18 January 2017
After publication of our article , we noticed some errors. In this manuscript, we amplified cDNA with 41.67 nmol/l RT primer and 70 nmol/l tagging primer for single-cell Quartz-Seq, and not pmol/l as originally stated. In addition, two mathematical expressions were inadvertently omitted.
Thus, in the section Whole-transcript amplification for single-cell Quartz-Seq, the following are correct:
Immediately after the second centrifugation, 0.8 μl of priming buffer (1.5× PCR buffer with MgCl2 (TaKaRa Bio), 41.67 nmol/l of the RT primer (HPLC-purified; Table 1), 4 U/μl of RNase inhibitor (RNasin Plus; Promega Corp., Madison, WI, USA), and 50 μmol/l dNTPs were added to each tube.
We then added 23 μl of the second-strand buffer (1.09× MightyAmp Buffer v2 (TaKaRa), 70 nmol/l tagging primer (HPLC-purified; Table 1), and 0.054 U/μl MightyAmp DNA polymerase (TaKaRa)) to each tube.
In the section Bioinformatics analysis, the equations should appear as follows:
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