Fig. 4
From: Tracing the origin of disseminated tumor cells in breast cancer using single-cell sequencing
Tracing the origins of DTCs in the breast cancer phylogenetic trees. Copy number-based phylogenetic trees drawn up for patients MicMa003 (a), MicMa083 (b), and MicMa107 (c). Nodes in the trees correspond to (sub)clones and are color-coded based on their type as indicated (primary or lymph node, DTC, AU, or normal cell). Grey nodes are not observed directly but can be inferred from the data. Nodes are annotated with their specific CNA event and, where possible, with their estimated cancer cell fraction (the percentage of tumor cells containing the indicated aberration). Within the tumors, branch lengths reflect differences in cancer cell fraction. The most recent common ancestor (MRCA) in each bulk sample is indicated with a thicker stroke. For MicMa107, single cells isolated 3 years post-diagnosis are represented as striped nodes. DTC 107M has undergone a whole-genome duplication (WGD)