Fig. 2

The secretome of pollen tubes grown through the pistil is unique from that of in vitro germinated pollen tubes and differs significantly from their respective proteomes. All sample types used for comparisons are representative of four replicates. a Three-dimensional principle component analysis of pollen tube secretomes (PS) and pollen tube proteomes (PP) from semi-in vivo (SIV) and 24 h in vitro germinated (PT24) pollen tubes. b Four-way Venn diagram of all sample types showing limited overlap between secretomes and proteomes but a relatively closer overlap between proteomes of SIV and in vitro germinated pollen tubes. c Pairwise comparison of sample similarities demonstrates that the secretome of pollen tubes grown through the pistil is unique from that of in vitro germinated pollen tubes and the pollen tube secretome is different from their respective pollen tube total proteomes, suggesting that regulated exo- and endocytosis occurs during pollen tube protein secretion. d GO term comparison of SIV and in vitro pollen tube secretomes, highlighting enriched activities where they overlap and protein accessions unique between the two sample types