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Fig. 6 | Genome Biology

Fig. 6

From: Characterization of chromatin accessibility with a transposome hypersensitive sites sequencing (THS-seq) assay

Fig. 6

THS-seq and ATAC-seq peak capture preferences and biases. All datasets and replicates were down-sampled to 8,351,125 unique alignments before analysis. a Total percentage of unique alignments in peaks out of the 8,351,125 unique alignments for each dataset. b The percentage of alignments that are in the larger 30 % of peaks called significant for each individual sample and replicate. c The percentage of alignments that are in the smaller 70 % of peaks called significant for each individual sample and replicate. d-f For all peaks in each individual dataset, the normalized number of alignments in each peak length, with peak lengths in increments of 100 base pairs, represented by mean ± SEM in (d) 100-cell THS-seq/Tn5059 data and 500-cell ATAC-seq/EzTn5 data, (e) 500-cell THS-seq/EzTn5 data and 500-cell ATAC-seq/Tn5059 data, and (f) 100-cell THS-seq/Tn5059 data and published 50,000-cell ATAC-seq data. Some data points were excluded from the graphs because values were beyond the axis, and the number of data points excluded for each graph is: (d) 13, (e) 16, and (f) 8

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