Genomic legacy of the African cheetah, Acinonyx jubatus
- Pavel Dobrynin†1,
- Shiping Liu†2, 25,
- Gaik Tamazian1,
- Zijun Xiong2,
- Andrey A. Yurchenko1,
- Ksenia Krasheninnikova1,
- Sergey Kliver1,
- Anne Schmidt-Küntzel15,
- Klaus-Peter Koepfli1, 3,
- Warren Johnson3,
- Lukas F.K. Kuderna4,
- Raquel García-Pérez4,
- Marc de Manuel4,
- Ricardo Godinez5,
- Aleksey Komissarov1,
- Alexey Makunin1, 11,
- Vladimir Brukhin1,
- Weilin Qiu2,
- Long Zhou2,
- Fang Li2,
- Jian Yi2,
- Carlos Driscoll6,
- Agostinho Antunes7, 8,
- Taras K. Oleksyk9,
- Eduardo Eizirik10,
- Polina Perelman11, 12,
- Melody Roelke13,
- David Wildt3,
- Mark Diekhans14,
- Tomas Marques-Bonet4, 24, 25,
- Laurie Marker16,
- Jong Bhak17,
- Jun Wang18, 19, 20, 21,
- Guojie Zhang2, 26 and
- Stephen J. O’Brien1, 22Email author
© Dobrynin et al. 2015
Received: 22 July 2015
Accepted: 17 November 2015
Published: 10 December 2015
Patterns of genetic and genomic variance are informative in inferring population history for human, model species and endangered populations.
Here the genome sequence of wild-born African cheetahs reveals extreme genomic depletion in SNV incidence, SNV density, SNVs of coding genes, MHC class I and II genes, and mitochondrial DNA SNVs. Cheetah genomes are on average 95 % homozygous compared to the genomes of the outbred domestic cat (24.08 % homozygous), Virunga Mountain Gorilla (78.12 %), inbred Abyssinian cat (62.63 %), Tasmanian devil, domestic dog and other mammalian species. Demographic estimators impute two ancestral population bottlenecks: one >100,000 years ago coincident with cheetah migrations out of the Americas and into Eurasia and Africa, and a second 11,084–12,589 years ago in Africa coincident with late Pleistocene large mammal extinctions. MHC class I gene loss and dramatic reduction in functional diversity of MHC genes would explain why cheetahs ablate skin graft rejection among unrelated individuals. Significant excess of non-synonymous mutations in AKAP4 (p<0.02), a gene mediating spermatozoon development, indicates cheetah fixation of five function-damaging amino acid variants distinct from AKAP4 homologues of other Felidae or mammals; AKAP4 dysfunction may cause the cheetah’s extremely high (>80 %) pleiomorphic sperm.
The study provides an unprecedented genomic perspective for the rare cheetah, with potential relevance to the species’ natural history, physiological adaptations and unique reproductive disposition.
KeywordsGenetic diversity Conservation biology Population biology
The African cheetah—the world’s fastest land animal—is a paradigm of physical prowess that displays numerous physiological adaptations allowing for magnificent high-speed sprints across the African plains. Cheetahs have elongated legs, slim aerodynamic skulls and enlarged adrenal glands, liver and heart, plus semi-retractable claws that grip the earth like football cleats as they race after prey at >100 km/hour. Cheetahs have captured the imagination of artists, writers, regal potentates and wildlife lovers for centuries. Initially descended from early Pliocene precursors related to American pumas, their fossil record extends across the Americas, Europe and Asia until the late Pleistocene (∼10,000–12,000 years ago) when an abrupt extinction after the last glacial retreat extirpated ∼40 species of large mammals, including cheetahs and pumas from North America [1–5].
Modern cheetahs range across eastern and southern Africa (a small number are in Iran, a relict of the Asiatic cheetah subspecies ) and are considered highly endangered by wildlife authorities and governments. As a species, cheetahs show a dramatic reduction in overall genetic variation revealed by multiple genomic markers, including an ability to accept reciprocal skin grafts from unrelated cheetahs [7–9]. Their genetic depletion correlates with elevated juvenile mortality, extreme abnormalities in sperm development, difficulties until recently in achieving sustainable captive breeding, and increased vulnerability to infectious disease outbreaks [10–13]. Cheetahs today remain a conservation icon and a symbol for the cost of genetic impoverishment caused by demographic reduction, close inbreeding and near extinction in small free-ranging natural populations. Genetic loss in modern cheetahs has been debated, validated and researched on multiple levels, and is believed to derive from one or more severe population bottlenecks that occurred over time and space during the Pleistocene epoch [7, 14–18]. That precipitous drop in number and genetic diversity, aggravated by behavioral reinforcement of immense range boundaries, led to the genetically depleted cheetah populations surviving today.
Here we present a detailed annotation and analysis of the assembled whole-genome sequence of African cheetah that affirms the genome-wide reduction of cheetah diversity and identifies gene adaptations that occurred in the cheetah’s evolutionary lineage.
Assembly and annotation of the cheetah genome
Genome sequence and assembly
A. jubatus raineyii (Tanzania)
75 × reference
A. jubatus jubatus (Namibia)
5 × resequencing
SOAP deNovo assembly
Tables S1, S4
Assisted assembly with domestic cat Fca-6.2
Fca-6.2 framework anchors:
a. Radiation Hybrid map
b. Linkage map
Estimated genome size (assembly and 17-mer)
Average GC content
Non-coding RNA 200,045 loci
a. 43,878 microRNA
b. 1,605 small nuclear RNA
c. 154,031 transport RNA
d. 531 ribosomal RNA
Single nucleotide variants (SNVs)
Tables S6, S7
39.48 % of cheetah genome
Complex tandem repeats
Genomic rearrangements of cheetah vs domestic cat
Figures S5, S6
Tables S13, S14
Nuclear mitochondrial segments
Positively selected genes
GARfield Genome Browser
Third, cheetah coding genes showed dramatic genetic diminution as great as 50-fold (∼98 %) relative to domestic cat or wildcat genome variation (Fig. 1 c). The extreme reduction in coding gene variants would explain the initial discovery of the cheetah’s depauperate genetic variation three decades ago with studies using allozymes, cellular protein electrophoretic variants and gene-based restriction fragment length polymorphism (RFLP) [7–9]. Fourth, cheetahs show on average 10–15-fold longer homozygous stretches relative to the feral domestic cat genome; on average 93 % of each cheetah’s genome was homozygous (Fig. 1 d; Additional file 1: Figure S8). Fifth, cheetah genomes show far less heterozygous SNV sites, 0.019–0.021 %, reduced to 50–61 % of the incidence in tigers, 30 % of humans and 15 % of domestic cats [19, 28] (Additional file 2: Tables S20 and S21). Sixth, complete mitochondrial genomes of cheetah similarly show on average 90 % reduction in SNVs relative to other species (Additional file 2: Table S25).
Seventh, we also investigated in detail the cheetahs’ major histocompatibility complex (MHC), a cluster of ∼280 immune-related genes, given their functional role and the remarkable observation that cheetahs accepted reciprocal skin allografts from unrelated individuals as if they were immunological “self” . An assisted assembly of 20 cheetah MHC sequence scaffolds on the domestic cat BAC library MHC assembly (total size 8.3 Mb) [29, 30] resolved 278 genes from extended class II, class II, class I and extended class I regions. Although most regions were well covered, complete homologues of certain class I MHC genes (FLA-I F, H and M) were not detected (Additional file 1: Figures S9 and S10; Additional file 2: Table S26). When we compared the structural organization and gene order of the MHC with other species, the cheetah and domestic cat were highly similar, but different from the dog and human. Cheetah and cat MHCs include three functional vomeronasal receptor genes (important for pheromone recognition ) in the extended class I region (these genes are absent in the human, nonhuman primates and dog). The cat and cheetah also displayed expansion of certain olfactory receptor genes (0.9 Mb and 30 genes) within the extended class I region . We compared the number of detected SNV variants (synonymous and non-synonymous) in the MHC immune genes from the cheetah (from Namibia and Tanzania), domestic cat, wildcat, human and dog [19, 20, 32]. We found a 95–98 % reduction in both populations of cheetahs and also for Cinnamon (a highly inbred Abyssinian cat who supplied the reference domestic cat genome Fca-6.2) [19, 20] relative to abundant SNVs in an outbred domestic cat (Boris), human and dog MHC regions (Fig. 2). The MHC-SNV reductions in the inbred cat and cheetah involve both synonymous and non-synonymous amino acid-altering substitutions. These numerous function-altering variants reflect a history of pathogen-based frequency-dependent selection driving MHC diversity higher across mammals (Additional file 2: Table S26) .
Model 4 (also denoted by 2D ISB), a two-dimensional (2D) model of an expanding ancestral population that subdivides into two bottlenecked derivative populations, showed the best fit based on low bootstrap variance and high maximum likelihood (LL=−43,587) (see “Materials and methods”; Additional file 1: Figure S12; Additional file 2: Table S27), as illustrated in Fig. 3. The DaDi modeling results imply a >100,000-year-old founder event for cheetahs, perhaps a consequence of their long Pleistocene migration history from North America across the Beringian land bridge to Asia, then south to Africa, punctuated by regular population reduction as well as limiting gene flow through territory protection. Alternatively, Barnett et al.  have postulated, based on a study of ancient DNA of Miracinonyx trumani (American cheetahs), that today’s African cheetahs originated from Asia, which would indicate that the 10,000-year-old founder effect coincided with an Asia to Africa cheetah dispersal around that time.
More recent late Pleistocene bottlenecks for eastern and southern African populations would further deplete variation in both populations [2, 7, 9]. The AFS modeling indicated a notable excess in derived alleles in the Namibian population compared to the Tanzanian population, implying historic gene flow from Namibian to Tanzanian predecessors estimated at >11,084–12,589 years ago in Africa (Fig. 3; Additional file 1: Figure S12; Additional file 2: Table S28). A parallel analysis using the pairwise sequentially Markovian coalescent (PSMC) algorithm for estimating demographic history lent support to the inference of decreasing cheetah population size in the last 100,000 years (Additional file 1: Figure S11).
A second approach used gene effect annotation in seven sequenced genomes to find harmful mutations segregated in cheetah populations. SNVs showing possible deleterious effects were identified using snpEff  and filtered with the names of 656 previously identified 1:1 orthologs from five species related to reproduction gene function and potentially harmful effects (e.g., stop codon gained and affected splice sites). A total of 61 genes were found and 20 of them (Additional file 3: Datasheet S8) showed a primary relationship to the reproductive abnormalities found in cheetahs. These mutations provide a valuable basis for association studies of reproductive impairments in cheetah populations.
The expanded genes were largely a variety of GO terms including olfactory and G-coupled protein receptors (also expanded in other Felidae [19, 20, 28]), which, if affirmed, would relate to cheetah physiology. For example, the LDH-A and LDH-B gene families showed twofold gene number expansions in certain Felidae (cat, cheetah and lion) compared to other mammals, which is potentially explanatory of the Felidae carnivorous life style (Additional file 1: Figure S14).
We searched for signatures of recent natural selection across all cheetah genes by assessing Dn/Ds ratios in alignments with orthologs from the lion, tiger, cat, human and mouse genomes. Specifically, we used the PAML branch-site test to test for positive selection along the cheetah phylogenetic lineage  and found 946 genes with significant signals (p<0.05 adjusted; Additional file 3: Datasheet S5), ten of which showed enrichment in specific GO terms. Five genes with signatures of selection were related to the regulation of cardiac and striated muscle contraction (ADORA1, RGS2, SCN5A, ADRA1 and CACNA1C); two genes (TAOK2 and ADORA1) were involved with MAPPK activity important in stress response, including heat stress, and four genes (APOC3, DDIT4, SUFU and PPARA) were associated with negative regulation of catabolic processes (Additional file 3: Datasheet S5). A copy number variation screen revealed 12.4 Mb included in segmental duplications (SDs) (shared among seven cheetahs) implicating gene regions and plausible gene candidates that might influence cheetah energetics, nutrition and sensory adaptations (Additional file 1: Figures S16 and S17; Additional file 2: Table S32; Additional file 3: Datasheet S7). These selected, expanded or duplicated genes are all possible explanatory candidates for mediating the cheetah’s adaptation to high-speed acceleration and short-term endurance.
African cheetah genomes display a remarkable reduction in endemic genetic variation and footprints of a fascinating natural history. Seven distinct measures show a species losing 90–99 % of variation levels seen in outbred mammals, well below that observed in genome studies of inbred dogs and inbred cats and in genetically depleted Tasmanian devil or Virunga mountain gorilla genomes (Figs. 1 and 2; Additional file 1: Figures S7–S10; Additional file 2: Tables S15–S25). A single exception, the Gir Forest lion population in Gujarat India, is a lion subspecies so inbred that DNA fingerprints of all Gir lions are identical (Fig. 1 b; [23, 24]). Cheetahs accept surgically exchanged skin grafts as if they were immunologic clones , prompting a study of the cheetah’s MHC. A high-resolution bacterial artificial chromosome (BAC) clone assembly of cat compared to cheetah directly revealed a loss of 2–4 MHC class I genes (FLA-F, -H, -I and -M) plus near zero class I amino acid variation across seven cheetah genomes, compared to appreciable domestic cat MHC diversity (Fig. 2; Additional file 1: Figures S9 and S10; Additional file 2: Table S26).
A coalescent demographic analysis (DaDi; ) plus a PSMC assessment of genome-wide SNV variation from two African cheetah populations show evidence of two bottlenecks: one ∼100,000 years BP and a second ∼12,000 years BP (Fig. 3). Previous mtDNA and microsatellite imputations also suggested a recent 10–12,000 years BP origin of modern cheetah variation, coincident with the late Pleistocene extinction of predominantly large animals: mammoths, mastodons, dire wolves, short-faced bears, American lions, saber-toothed tigers and four types of flesh-eating birds [1, 2, 25, 41]. Pumas and cheetahs also disappeared from North America at this time [4, 7]. We propose that the two late Pleistocene bottlenecks collapsed diversity in the cheetah’s ancestors and left behind signatures of demographic reduction in their genome sequence. First, ∼100,000 years ago, a migration of cheetahs across Asia and into Africa in a geographic spread possibly originating in North America [2, 4, 7, 8, 35] would have increased incestuous mating as a consequence of behavioral reinforcement of territories during these episodes. The more recent 12,000-year-old founding of African cheetah populations further reduced numbers and led to additional loss of endemic variability observed in modern cheetahs.
Genomic analysis revealed compelling statistical evidence for reproduction gene families accumulating excess functional (amino acid altering) variants in cheetahs, relative to other felids (Fig. 4) and identified ten fixed amino acid variants in the AKAP4 locus, a gene expressed exclusively in the testis whose homologues play a critical role in sperm development and onset of spermatozoa aberrations in several mammal species [42–44]. Five homozygous function-damaging mutations within AKAP4 likely would explain the very elevated pleiomorphic sperm (on average 81.6 % damaged spermatozoa) in every cheetah. Certain genes that mediate energy metabolism showed selective acceleration and are candidates for the cheetah’s adaptions to high-speed pursuit. Overall, the cheetah genome offers unparalleled insight into the history, adaptation and survival of a treasured endangered species. The zoo community’s assignment of captive cheetahs as research animals decades ago and the subsequent inclusion of genetic measures in nearly all conservation management deliberations illustrate the continuing benefit from the lessons of the cheetah [5, 10]. In concert with ecological, habitat restoration and other conservation issues, the cheetah’s genetic disposition should be useful in efforts to sustain and increase cheetah population numbers in their present and former range habitats .
Materials and methods
Sequencing and assembly of the Acinonyx jubatus genome
High molecular weight genomic DNA was extracted from blood or tissue samples of seven cheetahs, four from Namibia (one female and three males) and three from Tanzania (one female and two males), using the DNeasy Blood and Tissue kit (Qiagen). The genome of a male Namibian cheetah from the Cheetah Conservation Fund center (Chewbaaka) was sequenced at high coverage on the Illumina HiSeq2000 platform using a shotgun-sequencing approach. Extracted DNA was used to construct short, medium and long mate-pair libraries (170 bp, 500 bp, 800 bp, 2 kbp, 5 kbp, 10 kbp and 20 kbp). Statistics for the obtained reads are given in Additional file 2: Table S1. Six additional samples were sequenced at low coverage (5–6 ×) using 500 bp insert size libraries (Additional file 2: Table S2).
Sequence reads were assembled with SOAPdenovo2 , first into contigs and then iteratively into scaffolds with a total genome size of 2.38 Gb and scaffold N50 length of 3.1 Mb (contig N50 length of 28.2 kbp). The genome size was found to be smaller than that based on estimates of the 17-mer length distribution (Additional file 1: Figure S1; Additional file 2: Table S3) . This mismatch may be due to some repetitive sequences or highly complex regions that could not be assembled by the SOAPdenovo2 assembler (Additional file 2: Table S4).
We assessed the sequencing depth distribution and the GC content by mapping all the short insert-size reads back to the high-coverage reference genome and then calculating the GC content and depth for 10-kbp non-overlapping windows along the whole genome (Additional file 1: Figures S2 and S3).
To produce the cheetah chromosome assembly, we mapped cheetah scaffolds using NCBI BLAST  onto the domestic cat chromosomes from the Fca-6.2 assembly, which is based on previously published physical and linkage maps . A summary of the obtained cheetah chromosomes is given in Additional file 2: Table S5.
To find scaffolds that could be associated with the cheetah Y chromosome, we searched human genes located on the Y chromosome in the cheetah scaffolds that were not placed to the cat autosomes or X chromosome using our gene annotation pipeline (see “Annotation of Acinonyx jubatus genome” below). Of the 54 protein-coding genes on the human Y chromosome, sequences for 21 genes were predicted in the unplaced cheetah scaffolds (scaffold1492, scaffold1496, scaffold1636, scaffold803 and scaffold912). The SRY gene was predicted in the cheetah scaffold1636. In total, we found five scaffolds putatively constituting cheetah chromosome Y; their total length was 1,524,629 bp.
Annotation of Acinonyx jubatus genome
To identify all known Carnivora repeats, we used the RepeatMasker software  and the Repbase Update library  with the option to search for Carnivora-specific repeats. We searched for repeats in the following genomes: cheetah, lion (Panthera leo), tiger (Panthera tigris ), cat (Felis catus; the Fca-6.2 assembly ) and dog (Canis lupus familiaris; the CanFam3.1 assembly; ). A summary of the RepeatMasker results are given in Additional file 2: Table S6. In addition, we used the RepeatProteinMask tool, belonging to the RepeatMasker package, which identified transposable elements by aligning a genome sequence to a self-defined transposable-element protein database (Additional file 2: Table S7). To detect tandem repeats in five Carnivora genomes (cat, cheetah, dog, lion and tiger), we used the Tandem Repeats Finder (TRF) software, version 4.07  with the mismatch and maximum period parameters set to 5 and 2000. TRF output was processed as published previously .
Microsatellites with a monomer length less than 5 bp, including perfect microsatellites with a monomer length of less than 5 bp
Complex tandem repeats
Large tandem repeats characterized by large successfully assembled tandem repeat arrays that were divided into three subgroups by array length of 1, 3 and 10 kbp (Additional file 2: Tables S8 and S9)
The dog genome contains around 20 % more ascertained tandem repeats and significantly more assembled large tandem repeats in comparison with the four felid genomes.
Complex tandem repeats included large tandem repeats and satellite DNA characterized by GC content of arrays from 20 to 80 %, array length greater than 100 bp, copy number variations greater than 4 bp in length, array entropy greater than 1.76, monomer length greater than 4 bp, and imperfect tandem repeat array organization. Complex tandem repeats were classified into families by sequence similarity computed using NCBI BLAST according to the workflow from . Each family was named according to nomenclature based on the most frequent monomer length. The family Ajub483A is the most similar to the FA-SAT repeat of the domestic cat and it has predicted locations in the pericentromeric and pretelomeric regions [53, 54]. Families Ajub33A and Ajub113A have predicted locations in the pericentromeric regions. Family Ajub84A is based on the tandemly repeated zinc-finger motif (Additional file 2: Table S9).
In total, 20,343 protein-coding genes and 110,431 (10.1 Mb) non-coding RNA elements were identified in the cheetah genome (Additional file 2: Tables S10 and S11).
Coding genes To predict the protein-coding genes in the cheetah, we combined both homology-based and de novo gene prediction tools. We first downloaded the gene sets from Ensembl (http://www.ensembl.org)  for the cat, dog and human and chose the unique locus for each gene by extracting the longest open reading frame for the multi-open-reading-frame genes. We then used the NCBI BLAST tool  with an E-value cutoff of 10−5 for mapping all orthologous genes onto the reference cheetah genome in an effort to speed up alignment. We also used Genewise  to carry out local alignment and predict a gene structure for each possible linked orthology hit. Genes that were complete both in terms of structure and in length based on the orthology searches were then used as input to train the hidden Markov gene model to predict also gene structure using the Augustus software package . If a conflict was found between the orthology-based and de novo prediction methods, we used the gene result based on the orthology-based methods alone.
Non-coding RNA Identification of tRNA genes The tRNA genes were predicted by tRNAscan-SE  with eukaryote parameters. If more than 80 % length of a tRNA gene was covered by the transposable small interspersed elements (SINE), then it was defined as SINE-masked. The tRNA identity to human was calculated with a MUSCLE  global alignment.
Identification of rRNA genes The rRNA fragments were identified by aligning the rRNA template sequences from the human genome using BlastN  at E-value 10−5, with a cutoff of identity ≥85 % and match length ≥50 bp.
Identification of other ncRNA genes The miRNA and snRNA genes were predicted using the INFERNAL  software against the Rfam database (release 9.1, 1372 families)  with Rfam’s family-specific “gathering” cutoff. To accelerate the speed, we performed a rough filtering prior to INFERNAL by aligning the obtained miRBase predictions against the Rfam sequence database using Blastn under an E-value of 1. The miRNA predictions were first aligned against the mature sequences of human and dog from miRBase  (release 13), allowing one base mismatch, and then aligned against the precursor sequences, requiring more than 85 % overall identity. The snoRNA predictions were aligned to human H/ACA and C/D box snoRNAs and Cajal body-specific scaRNAs from snoRNABase  (version 3), and required a cutoff of 85 % overall identity. The spliceosomal RNA predictions were aligned to the Rfam sequence database, and required a cutoff of 90 % overall identity.
To increase the sample size (power) for genome variation and population analyses, we combined the reads from the six re-sequenced cheetah genomes with the reads from the reference cheetah genomes using only 500-bp insert size libraries for all individuals. Therefore, our population genomic analyses are based on seven individual cheetahs, four from Namibia and three from Tanzania.
An N-content of more than 10 %
More than 40 % of the read length was below Q7
Reads overlapping by more than 10 bp with an adapter sequence, with a maximum of 2 bp mismatches
Paired-end reads, which overlapped by more than 10 bp between the two ends
We observed that both ends of a read, with total length equal to 90 bp, always had low quality scores, especially the 3′ end. We, therefore, trimmed a maximum 10 bp off the 5′ end of a read if the consecutive quality score was less than Q20. Likewise, we trimmed a maximum of 40 bp off the 3′ end of a read if the consecutive quality score was less than Q20. In this way, we retained enough bases with high quality for the subsequent read mapping.
Alignments with a mapping quality score less than 20 (<MQ20)
Non-unique alignments, i.e., any alignments that mapped to multiple positions in the genome sequence
Duplicated alignments, i.e., two or more reads that aligned to exactly the same position in the genome sequence
SNV calling using the site frequency spectrum method SNV calling based on low-depth sequencing (< 10×) is a challenge for most of the current strategies. The method described in  is a robust and high-precision method for SNV calling at low depth based on the methodology of the site frequency spectrum (SFS). It uses a maximum likelihood algorithm to estimate the maximum probability for each site. We used the SOAPsnp method to produce the GLFv2 format for each site and then used ANGSD  and beagle  to extract the genotype.
Initially, we obtained the SNV list for high-coverage sites across the whole genome in which the minimum and maximum read depths for each sample were set to 5 × and 30 ×, respectively (Additional file 1: Figure S4). Finally, 3.44 million SNVs were ascertained (Additional file 2: Table S15). In addition, variant positions located in repeat regions were filtered out, which produced a final set of 1,820,419 SNVs, which is 53 % of the original SNV number (3,438,824). We ascertained the distribution of SNVs across the genome for all individuals (Additional file 2: Table S20). All SNV variants were annotated for each individual using snpEff  and a database was constructed from the annotated cheetah genes (Additional file 1: Tables S16–S18). For all observed SNVs, 73.7 % were located outside the protein-coding genes; only 1.3 % were inside exons and a major fraction of them, 24.92 %, were found inside introns (Additional file 2: Table S19).
Nuclear mitochondrial segments
We retrieved copies of nuclear mitochondrial segments using the whole Felis catus cytoplasmic mtDNA genome (RefSeq:NC_001700) as the query input sequence in an NCBI BLAST search. This search found 143 sequences with significant identity to the cat mtDNA genome, 50 of which contained complete mitochondrial genes and 93 partially covered genes (Additional file 2: Table S12).
Mitochondrial genome assembly and nucleotide diversity analysis
Complete mitochondrial genomes of all seven cheetahs were assembled using the 500-bp insert libraries from the reference and re-sequenced individuals. There is very little variation among the cheetah sequences (∼0.1–0.2 % divergence across the entire mitogenome). There does appear to be variation that separates the eastern versus southern African cheetah populations.
Additionally, nucleotide diversity was examined in a number of other mammalian species and compared with the cheetah. Cheetahs have the lowest numbers in diversity among other species, and numbers are correlated with population sizes for Tanzania and Namibia (Additional file 2: Table S25).
Whole-genome alignment We used the Progressive Cactus software [21, 67] to align the scaffold assemblies of the tiger, lion and cheetah, and the chromosome assemblies of the domestic cat (Fca-6.2) and domestic dog (CanFam3.1). The dog genome was included as an outgroup. The percentage alignment of the cheetah genome to the other genomes was 93.6 % for the tiger, 91.6 % for the lion, 91.1 % for the domestic cat and 74.1 % for the domestic dog.
Calculation of synteny blocks The multiple alignment was further processed with the GRIMM synteny algorithm [22, 68]. The aligned segments of the genomes are used as anchors that are further chained into syntenic blocks. The size of the blocks is a flexible quantity and can be controlled by the input parameters that correspond to the minimal size of a block and maximal proximity between the aligned anchors that will be joined into one cluster. We set both parameters equal to 300 kbp because other variants produced many short syntenic blocks and these parameter values were shown to be optimal in previous analyses of the human and mouse . For each synteny block, we calculated the density of anchors. Density is defined as the sum of lengths of aligned anchors divided by the length of the whole block . After filtering out those syntenic blocks that correspond only to single scaffolds in the cheetah genome, 93 syntenic blocks remained, which were used for further analyses. The ten longest syntenic blocks showing rearrangements are shown in Additional file 1: Figure S6.
Calculation of genome rearrangement scenarios We applied the GRIMM algorithm [22, 68] to the synteny blocks to calculate the rearrangement scenarios that occurred between the cheetah and each of the other four species. Since we used scaffold assemblies for the cheetah, tiger and lion, we needed to distinguish rearrangement events that occurred in the separate scaffolds from those that occurred within the scaffolds of each species. The synteny blocks between the cat and cheetah genomes cover the largest fraction of the cheetah genome (98.6 %) (Additional file 2: Table S13), likely because the domestic cat genome assembly is more complete compared to the assemblies of the lion and tiger. The results also agree with the relatively short evolutionary distance between the cat and the cheetah, 6.7 MY . For comparison, the synteny blocks in the human–mouse alignment cover 82 % of the human genome , where the divergence time for human and mouse is 96 MY.
We also analyzed the distribution of the syntenic block lengths for the blocks for which the length was greater than 10 kbp (Additional file 1: Figure S5). The peaks in the graph correspond to the number of synteny blocks with the corresponding length. The plots demonstrate that there are more syntenic blocks of shorter lengths than those of the longest one. We found that the lion genome is the most fragmented, which explains why most cheetah–lion synteny blocks have a length <1.5 Mb. The graphs for the cat and dog are similar, with syntenic blocks that are longer compared to the lion and tiger due to the higher assembly quality of the former two species. With the GRIMM software, we also calculated the rearrangement scenarios based on the multiple alignments (Additional file 2: Table S14). The results of this approach can be verified by PCR amplification.
Gene evolution in Acinonyx jubatus
Gene family clusters
Blastp was applied to align all protein sequences against a database containing a protein data set of all species, with the E-value set to 10−7 and with -outfmt 6. In addition, fragmented alignments were joined for each gene pair using Solar (perl solar.pl -a prot2prot -f m8 -z). We assigned a connection (edge) between two nodes (genes) if more than 1/3 of the region aligned to both genes. An Hscore that ranged from 0 to 100 was used to weight the similarity (edge). For two genes, G 1 and G 2, the Hscore was defined as s c(G 1,G 2)/ max(s c(G 1,G 1),s c(G 2,G 2)), where sc is the BLAST bit score.
Gene families were clustered using Hcluster_sg with options set to -w 10 -s 0.34 -m 500 -b 0.1. We used the average distance for the hierarchical clustering algorithm, requiring the minimum edge weight (Hscore) to be larger than 5, and the minimum edge density (total number of edges/theoretical number of edges) to be larger than 1/3. Clustering for a gene family would stop if it already had one or more of the outgroup genes.
To determine the expansion and contraction of the orthologous protein families among nine mammalian species, we used CAFE 3.0  with its lambda option (the gene gain and loss rate) set to 0.0024. GO enrichment analyses were used to test for overrepresented functional categories among expanded genes and genome-background genes (Additional file 3: Datasheets S3 and S4). All results with a p value higher than 10−4 were filtered out. Also the false discovery rate was calculated to take into account multiple testing.
Positively selected genes
To detect genes that evolved under positive selection, we used PAML, a maximum-likelihood method for analysis of molecular evolution [37, 74]. Specifically, we used PAML’s branch-site test  to test for positive selection along the cheetah lineage. We compared model A1, in which sites may evolve neutrally and under purifying selection with model A, which allows sites to be also under positive selection. p values were computed using the χ 2 statistic adjusted using the false discovery rate  to allow for multiple testing. Alignment quality is of major importance for studies of positive selection, as alignment errors can lead to unacceptably high false positives using the branch-site model . We used PRANK , which differs from other alignment tools in that it utilizes evolutionary information in determining where to place a gap. Studies of the branch-site test and other PAML models support PRANK to be the alignment tool of choice [77, 79]. We filtered the PRANK alignments by Gblocks [80, 81] and excluded genes with sequence properties that often lead to false positives, such as genes with a high proportion of low complexity or disordered regions, ubiquitous domains, repeats, transmembrane and coiled-coil regions, overlapping domains, uncharacterized proteins, collagens, Zn-finger proteins, olfactory receptors and other large families or clustered arrangements. We identified 947 genes (Additional file 3: Datasheet S5) under positive selection (p<0.05 adjusted for multiple testing). Of the 947 genes that showed signals of positive selection in the cheetah lineage, seven genes were selected during GO analysis (the maximum p value was 10−3), which we found were related to regulation of muscle contraction (ADORA1, RGS2, SCN5A, ADRA1B, CACNA1C, TAOK2 and SCAI), and which exhibit an important role in cheetah locomotion and cardiac muscle contraction, and two genes (TAOK2 and ADORA1) associated with MAPKK activity, which is important in the stress response, including heat stress (Additional file 3: Datasheet S5).
Analysis of reproduction-related gene families in Acinonyx jubatus
To analyze reproduction-related genes in the cheetah genome, we obtained human genes belonging to the gene ontology term GO:0000003 (Reproduction) from the Ensembl Genes database . A total of 1730 transcripts of 964 protein-coding genes were obtained. This set was used to find 1:1 orthologous genes in the cheetah, cat, tiger, dog and human. To find orthologous relationships between genes, the method Proteinortho/PoFF , which utilizes both BLAST alignment and synteny approaches, was used. Of the 1730 transcripts, the search resulted in 656 1:1 orthologs for the five species.
Orthologs were aligned using the parallel tool ParaAT  with the MAFFT aligner  with the options set to the most accurate, taking into account absent exons in some genes. To delete putatively misaligned regions, Gblocks  was applied to the multiple sequence alignments with stringent filtering criteria; the following Gblocks parameters were used: -b1=5 -b2=4 -b3=6 -b4=10 -b5=h.
We used PAML to find genes with an accelerated accumulation of non-synonymous to synonymous rates (Dn/Ds) in the cheetah lineage relative to the mean in the four species. An accelerated accumulation of non-synonymous substitutions may indicate an increased number of moderate and deleterious mutations that are harmful for the reproductive physiology in the cheetah lineage. To estimate the rate of non-synonymous mutation accumulation, the free-ratio model implemented in PAML was used . The model assumes a different lineage-specific rate of the Dn/Ds ratio for each branch of the tree. All genes were concatenated into one “supergene” and Dn/Ds was estimated for each species. Surprisingly, the cheetah had the highest values for Dn/Ds rate among the five studied species. To test this effect further, a new data set was generated using 500 bootstrap replications (Fig. 4 a).
To test the hypothesis that there are elevated Dn/Ds values in the cheetah lineage, the total set of 6348 genome-wide orthologs was constructed for all genes from the following species: cat, tiger, cheetah and dog. After filtering unreliably aligned regions using Gblocks and concatenation, a 10-Mb long alignment of coding sequences was obtained. Based on the alignment, 200 bootstrap replications were performed and the resulting data set was used for the free-ratio analysis in PAML. For the whole genome data set, the same results as given above were obtained (Fig. 4 b); the cheetah had accelerated Dn/Ds ratio values relative to the other species.
M0—Same Dn/Ds for all branches of the tree
M2—Different Dn/Ds for background (cat, human, dog and tiger) and foreground (cheetah) branches
All genes with Dn/Ds ratio values in the cheetah branch greater than those in the other branches based on the M2 model were retrieved from the whole data set and the likelihood ratio test between the M0 and M2 models was performed (to test the hypothesis that the Dn/Ds ratio is significantly greater in the cheetah lineage compared to the other species). In total, 92 genes with p<0.05 were obtained (Additional file 3: Datasheet S6). These genes were manually screened using public databases (GeneCards and Ensembl) to find genes directly related to spermatogenesis, azoospermia, oligospermia, oogenesis and gonadal dysfunction. A final list of 18 genes was used (Additional file 2: Table S29) to search the genetic-disease databases, including OMIM, KEGG and MalaCards, as well as to screen for all non-synonymous mutations and deletions. The pathogenicity of mutations was assessed using PolyPhen2  with human proteins as the model for the cheetah. Among the 18 genes, we discovered one gene that showed an excess of possibly damaging missense mutations and was related to important spermatogenesis functions: AKAP4. We used Sanger sequencing to validate AKAP4 mutations in 10 Namibian cheetahs. Four from the five non-synonymous substitutions were confirmed in 9 samples and appeared to be homozygous. The fifth mutation was not detectable as it was located in one of the primer sequences.
Analyses of genetic diversity in the Acinonyx jubatus genome
SNV diversity was analyzed for the seven cheetahs and compared with SNV diversity in four other species: domestic cat, Bengal tiger, Siberian tiger and African lion. We constructed 50-kbp windows from the 3802 cheetah scaffolds, which were used to estimate SNV density at each window. Of these, 2386 scaffolds had lengths less than the specified window size and thus, were excluded from further analysis; most of these fragments were contigs with length less than 500 bp. The remaining 46,787 windows used had a total length of 2.34 Gb. Altogether, the windows constituted 99.12 % of the total length of the genome. The number of genes with SNVs located in the coding sequences (exons) was also examined for SNV density and compared among species (Additional file 2: Tables S20–S24).
Runs of homozygosity were estimated following the method described in  and using PLINK with the following parameters: –homozyg-window-snp 20 –homozyg-density 50 –homozyg-kb 10. Genome-wide heterozygosity was estimated by splitting the whole genome into non-overlapping windows of 100 kbp and counting the number of SNVs in them. Next, a window was considered heterozygous if the number of SNVs in it was greater than 40, otherwise it was considered homozygous. In Additional file 1: Figures S8a–S8d, the distribution of homozygous and heterozygous windows is shown for Boris (an outbred domestic cat), Cinnamon (an inbred domestic cat), Chewbaaka (a cheetah) and the mountain gorilla individual , respectively.
Demographic history analyses of the Acinonyx jubatus population
Pairwise sequentially Markovian coalescent analysis
We used the PSMC method  to infer the effective population size trajectory through time of the high-coverage cheetah genome (Chewbaaka). We used the Burrows–Wheeler aligner  and samtools  for mapping and genotyping. The generation time was set to 3 years and the mutation rate to 0.3×10−8, which was based on the whole-genome alignment between the cheetah and domestic cat generated using LASTZ  and calculating the number of differences between the two species and dividing by their divergence time (7 MY).
The PSMC results showed a gradual reduction in effective population size through time without any evidence for a sharp bottleneck (Additional file 1: Figure S11). These results may be due to the PSMC analysis having lower sensitivity for events during the more recent past and/or that any bottleneck event was short and severe, leaving little or no trace in the genome.
Diffusion approximation for demographic inference (DaDi) analysis
For the two cheetah populations analyzed (southern in Namibia and eastern in Tanzania), the AFS corresponds to a multidimensional matrix X, where each x ij entry gives the number of SNVs with an observed derived allele count of i in population 1 (Namibia) and j in population 2 (Tanzania). The likelihood is computed, given the expected AFS under a given evolutionary model. Each entry in the expected AFS reflects the probability of a given SNV falling into that cell. Assuming that all SNVs are n (that is, assuming free recombination between SNVs), these probabilities can be derived from the distribution of allele frequencies of each population, which in turn can be found with diffusion approximations of evolutionary processes, such as the size and timing of demographic changes.
To infer the demographic history of the two cheetah populations, we used the DaDi tool . Briefly, DaDi can generate a site AFS under one or more demographic scenarios. The aim is then to maximize the similarity between the expected allele frequency and the observed SFS over the parameter value space. Fitting can be evaluated by computing a composite-likelihood among different demographic scenarios.
The ancestral population splits into two subpopulations followed by limited migration from one subpopulation to another (2D IM model).
The ancestral population first undergoes a bottleneck, followed by splitting into two subpopulations (2D BIM model).
The ancestral population first splits into two subpopulations followed by a bottleneck event and then there is expansion/recovery of each subpopulation (2D SBR model).
The ancestral population first grows in size for ∼100,000 years prior to splitting into two subpopulations, followed by an independent bottleneck in each subpopulation (2D ISB model).
Segmental duplication analysis in the Acinonyx jubatus genome
To estimate regions of recent SDs from the genomes of six Acinonyx jubatus individuals, we applied an approach based on genome-wide differences of depth of coverage .
Reference assembly preparation Regions detected by RepeatMasker  and TRF  were masked to remove most of the repetitive regions present in the assembly. We further sought to identify and mask potential hidden repeats by using a kmer-based approach. Scaffolds and contigs were partitioned into kmers of 36 bp (with adjacent kmers overlapping by 5 bp) and these kmers were mapped to the assembly using mrsFast  to account for multi-mappings. Overrepresented kmers, defined as those with three or more mappings into the assembly, were additionally masked (Additional file 1: Figure S15; Additional file 2: Table S31). For subsequent analysis, we created a shortened version of the assembly that did not include scaffolds or contigs below 10 kbp since we require SDs to expand at least this length because of the lower coverage of the genomes.
Read mapping and detection of copy number variation After checking the overall quality of the raw sequencing data, we split the reads into two consecutive kmers of 36 bp corresponding to positions 10–46 and 46–81. We chose the offsets in such way to trim regions of potentially lower-quality reads. These kmers where then mapped with mrFast  to the cheetah scaffolds masked by RepeatMasker and TRF (Additional file 2: Table S31) on which an additional 36 bp flanking each masked segment (referred to as padding regions below) were masked. The reason for the introduction of additional padding regions is that copy number variations are detected using mrCaNaVar  via the read depth in non-overlapping windows of 1 kbp of the unmasked sequence; i.e., the genomic coordinates of these windows may exceed 1 kbp, as they include masked regions. Reads originating from a region that overlaps a masked segment will not be mappable onto the genome and might, therefore, lead to a drop-off in estimates of the coverage of those positions. To avoid this bias, paddings the size of a split read were introduced.
A genome-wide read depth distribution was calculated by iteratively excluding windows with the most extreme read depth (RD) values and retaining the remaining windows as control regions. The copy number (CN) of any given window was then calculated as CN=2×RD/ mean(RD in control regions). The distribution of copy number values in control regions centered then to the value of 2 (Additional file 1: Figure S16).
Calling duplication blocks We define an SD as a region constituted of at least five consecutive windows of a non-overlapping non-masked sequence with CN > mean CN(control regions) + 3 standard deviations, allowing for one of those windows to have a CN value above mean + 2 standard deviations. The cutoffs were defined per sample. Additionally, these windows were to span at least 10 kbp in genomic coordinates. Furthermore, regions with an absolute copy number above 100 in any sample were excluded. For the downstream analysis, we additionally excluded gaps from the called intervals. Furthermore, we did not consider scaffolds that putatively derive from sex chromosomes.
We found a total of 7.8 Mb of the cheetahs’ autosomal genome to be composed of SDs across the six analyzed genomes. Duplicated regions for each individual range from 4.4 to 5.4 Mb and are summarized in Additional file 2: Table S32. About half of these regions (2.4 Mb) are shared by all individuals, despite the relatively low coverage, which may decrease our power to detect SDs. Still, these numbers are still reasonably similar to the ones reported for the domestic cat (9.1 Mb in duplications and 4.3 Mb in shared duplications) .
We intersected shared duplicated regions with gene annotations, requiring at least 60 % of the feature to overlap the duplications to be considered. In this way, we identified coding sequences of 173 predicted genes fixed as potential duplications in all individuals. A full list of the identifiers can be found in Additional file 3: Datasheet S7. An example of a fixed duplication intersecting coding regions can be found in Additional file 1: Figure S17. We performed a simple online GO-term enrichment analysis (http://amigo.geneontology.org/rte) with the human parent identifiers or the human orthologs of parent identifiers of genes in fixed duplication and found ontologies associated with smell, sensory perception, stimulus detection and catabolic processes to be enriched.
Software used in study
Availability of supporting data
The data can be accessed through BioProject accession numbers PRJNA297632 for the whole-genome sequence and PRJNA297824 for the re-sequence data. The SRA for whole-genome sequencing can be accessed via reference numbers: SRR2737512, SRR2737513, SRR2737514, SRR2737515, SRR2737516, SRR2737517, SRR2737518, SRR2737519, SRR2737520, SRR2737521, SRR2737522, SRR2737523, SRR2737524, SRR2737525, SRR2737526, SRR2737527, SRR2737528, SRR2737529, SRR2737530, SRR2737531, SRR2737532, SRR2737533, SRR2737534, SRR2737535, SRR2737536, SRR2737537, SRR2737538, SRR2737539, SRR2737540, SRR2737541, SRR2737542, SRR2737543, SRR2737544, SRR2737545. This whole-genome shotgun project has been deposited at DDBJ/EMBL/GenBank under the accession LLWD00000000. The version described in this paper is LLWD01000000.
This work was supported in part by a Russian Ministry of Science Mega-grant (no 11.G34.31.0068), a St. Petersburg State University grant (no 1.50.1623.2013), an ICREA grant (no. BFU2014-55090-P), an EMBO YIP 2013 grant (no. BFU2015-7116-ERC) and an MICINN grant (no. BFU2015-6215-ERC). Sample collection and validation of reproductive genes were performed under the permit number 1833/2013, granted by the Namibian Ministry of Environment and Tourism. The authors would like to express their gratitude to Benedict Paten, Joel Armstrong, Glenn Hickey and Brian Raney of the UCSC Genomics Institute for their support of the Progressive Cactus tool and the HAL tools package.
Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
- Hewitt G. The genetic legacy of the Quaternary ice ages. Nature. 2000; 405(6789):907–13.View ArticlePubMed
- Werdelin L, Yamaguchi N, Johnson WE, O’Brien SJ. Phylogeny and evolution of cats (Felidae) In: Macdonald D, Loverage A, editors. Biology and conservation of wild felids. Oxford: Oxford University Press: 2010. p. 59–82.
- Neff NA. The big cats: the paintings of Guy Coheleach. New York: Abradale Press/Abrams; 1986.
- Culver M, Johnson WE, Pecon-Slattery J, O’Brien SJ. Genomic ancestry of the American puma (Puma concolor). J Hered. 2000; 91(3):186–97.View ArticlePubMed
- Marker L, Eszterhas S. A future for cheetahs: Cheetah Conservation Fund; 2014. ISBN-13: 978-0615933207.
- Charruau P, Fernandes C, Orozco-terWengel P, Peters J, Hunter L, Ziaie H, et al. Phylogeography, genetic structure and population divergence time of cheetahs in Africa and Asia: evidence for long-term geographic isolates. Mol Ecol. 2011; 20(4):706–24.PubMed CentralView ArticlePubMed
- O’Brien SJ, Johnson WE. Big cat genomics. Annu Rev Genomics Hum Genet. 2005; 6:407–29.View ArticlePubMed
- O’Brien SJ, Wildt DE, Goldman D, Merril CR, Bush M. The cheetah is depauperate in genetic variation. Science. 1983; 221(4609):459–62.View ArticlePubMed
- O’Brien SJ, Roelke ME, Marker L, Newman A, Winkler CA, Meltzer D, et al. Genetic basis for species vulnerability in the cheetah. Science. 1985; 227(4693):1428–34.View ArticlePubMed
- Marker L, O’Brien SJ. Captive breeding of the cheetah (Acinonyx jubatus) in North American zoos (1871–1986). Zoo Biol. 1989; 8(1):3–16.View Article
- Wildt DE, Bush M, Howard JG, O’Brien SJ, Meltzer D, Van Dyk A, et al. Unique seminal quality in the South African cheetah and a comparative evaluation in the domestic cat. Biol Reprod. 1983; 29(4):1019–25.View ArticlePubMed
- Crosier AE, Marker L, Howard J, Pukazhenthi BS, Henghali JN, Wildt DE. Ejaculate traits in the Namibian cheetah (Acinonyx jubatus): influence of age, season and captivity. Reprod Fertil Dev. 2007; 19(2):370–82.View ArticlePubMed
- Heeney JL, Evermann JF, McKeirnan AJ, Marker-Kraus L, Roelke ME, Bush M, et al. Prevalence and implications of feline coronavirus infections of captive and free-ranging cheetahs (Acinonyx jubatus). J Virol. 1990; 64(5):1964–72.PubMed CentralPubMed
- May RM. Population genetics. The cheetah controversy. Nature. 1995; 374(6520):309–10.View ArticlePubMed
- Caro TM, Laurenson MK. Ecological and genetic factors in conservation: a cautionary tale. Science. 1994; 263(5146):485–6.View ArticlePubMed
- Merola M. A reassessment of homozygosity and the case for inbreeding depression in the cheetah, Acinonyx jubatus: implications for conservation. Conserv Biol. 1994; 8(4):961–71.View Article
- O’Brien SJ. Intersection of population genetics and species conservation: the cheetah’s dilemma In: Hecht MK, Macintyre RJ, Clegg MT, editors. Evolutionary biology. New York: Plenum Press: 1998. p. 79–91.
- Castro-Prieto A, Wachter B, Sommer S. Cheetah paradigm revisited: MHC diversity in the world’s largest free-ranging population. Mol Biol Evol. 2011; 28(4):1455–68.View ArticlePubMed
- Tamazian G, Simonov S, Dobrynin P, Makunin A, Logachev A, Komissarov A, et al. Annotated features of domestic cat—Felis catus genome. GigaScience. 2014; 3(1):13.PubMed CentralView ArticlePubMed
- Montague MJ, Li G, Gandolfi B, Khan R, Aken BL, Searle SM, et al. Comparative analysis of the domestic cat genome reveals genetic signatures underlying feline biology and domestication. Proc Natl Acad Sci. 2014; 111(48):17230–35.PubMed CentralView ArticlePubMed
- Paten B, Earl D, Nguyen N, Diekhans M, Zerbino D, Haussler D. Cactus: algorithms for genome multiple sequence alignment. Genome Res. 2011; 21(9):1512–28.PubMed CentralView ArticlePubMed
- Pevzner P, Tesler G. Genome rearrangements in mammalian evolution: lessons from human and mouse genomes. Genome Res. 2003; 13(1):37–45.PubMed CentralView ArticlePubMed
- Wildt DE, Bush M, Goodrowe KL, Packer C, Pusey AE, Brown JL, et al. Reproductive and genetic consequences of founding isolated lion populations. Nature. 1987; 329(6137):328–31.View Article
- Gilbert D, Packer C, Pusey A, Stephens J, O’Brien S. Analytical DNA fingerprinting in lions: parentage, genetic diversity, and kinship. J Hered. 1991; 82(5):378–86.PubMed
- Driscoll CA, Menotti-Raymond M, Nelson G, Goldstein D, O’Brien SJ. Genomic microsatellites as evolutionary chronometers: a test in wild cats. Genome Res. 2002; 12(3):414–23.PubMed CentralView ArticlePubMed
- Shankaranarayanan P, Banerjee M, Kacker RK, Aggarwal RK, Singh L. Genetic variation in Asiatic lions and Indian tigers. Electrophoresis. 1997; 18(9):1693–700.View ArticlePubMed
- O’Brien SJ, Martenson JS, Packer C, Herbst L, de Vos V, Joslin P, et al. Biomedical genetic variation in geographic isolates of African and Asiatic lions. Natl Geogr Res. 1987; 3(1):114–24.
- Cho YS, Hu L, Hou H, Lee H, Xu J, Kwon S, et al. The tiger genome and comparative analysis with lion and snow leopard genomes. Nat Commun. 2013;4.
- Yuhki N, Beck T, Stephens RM, Nishigaki Y, Newmann K, O’Brien SJ. Comparative genome organization of human, murine, and feline MHC class II region. Genome Res. 2003; 13(6a):1169–79.PubMed CentralView ArticlePubMed
- Yuhki N, Beck T, Stephens R, Neelam B. O’Brien SJ. Comparative genomic structure of human, dog, and cat MHC: HLA, DLA, and FLA. J Hered. 2007; 98(5):390–9.View ArticlePubMed
- Keverne EB. The vomeronasal organ. Science. 1999; 286(5440):716–20.View ArticlePubMed
- Lindblad-Toh K, Wade CM, Mikkelsen TS, Karlsson EK, Jaffe DB, Kamal M, et al. Genome sequence, comparative analysis and haplotype structure of the domestic dog. Nature. 2005; 438(7069):803–19.View ArticlePubMed
- Cagliani R, Sironi M. Pathogen-driven selection in the human genome. Int J Evol Biol. 2013:2013.
- Gutenkunst RN, Hernandez RD, Williamson SH, Bustamante CD. Inferring the joint demographic history of multiple populations from multidimensional SNP frequency data. PLoS Genet. 2009; 5(10):e1000695.PubMed CentralView ArticlePubMed
- Barnett R, Barnes I, Phillips MJ, Martin LD, Harington CR, Leonard JA, et al. Evolution of the extinct sabretooths and the American cheetah-like cat. Curr Biol. 2005; 15(15):R589—90.View ArticlePubMed
- Fitzpatrick JL, Evans JP. Reduced heterozygosity impairs sperm quality in endangered mammals. Biol Lett. 2009; 5:320–3.PubMed CentralView ArticlePubMed
- Yang Z. PAML 4: phylogenetic analysis by maximum likelihood. Mol Biol Evol. 2007; 24(8):1586–91.View ArticlePubMed
- Zhang Z, Xiao J, Wu J, Zhang H, Liu G, Wang X, et al. ParaAT: a parallel tool for constructing multiple protein-coding DNA alignments. Biochem Biophys Res Commun. 2012; 419(4):779–81.View ArticlePubMed
- Cingolani P, Platts A, Wang LL, Coon M, Nguyen T, Wang L, et al. A program for annotating and predicting the effects of single nucleotide polymorphisms, SnpEff: SNPs in the genome of Drosophila melanogaster strain w1118; iso-2; iso-3. Fly. 2012; 6(2):80–92.PubMed CentralView ArticlePubMed
- Jones P, Binns D, Chang HY, Fraser M, Li W, McAnulla C, et al. InterProScan 5: genome-scale protein function classification. Bioinformatics. 2014; 30(9):1236–40.PubMed CentralView ArticlePubMed
- Menotti-Raymond M, O’Brien SJ. Dating the genetic bottleneck of the African cheetah. Proc Natl Acad Sci. 1993; 90(8):3172–6.PubMed CentralView ArticlePubMed
- Brown PR, Miki K, Harper DB, Eddy EM. A-kinase anchoring protein 4 binding proteins in the fibrous sheath of the sperm flagellum. Biol Reprod. 2003; 68(6):2241–8.View ArticlePubMed
- Miki K, Eddy EM. Identification of tethering domains for protein kinase A type I α regulatory subunits on sperm fibrous sheath protein FSC1. J Biol Chem. 1998; 273(51):34384–90.View ArticlePubMed
- Carr D, Newell A. The role of A-kinase anchoring proteins (AKaps) in regulating sperm function. Soc Reprod Fertil Suppl. 2006; 63:135–41.
- Frankham R, Ballou JD, Briscoe DA. Introduction to conservation genetics: Cambridge University Press; 2002. ISBN-13: 978-0521702713.
- Luo R, Liu B, Xie Y, Li Z, Huang W, Yuan J, et al. SOAPdenovo2: an empirically improved memory-efficient short-read de novo assembler. GigaScience. 2012; 1(1):18.PubMed CentralView ArticlePubMed
- Liu B, Shi Y, Yuan J, Hu X, Zhang H, Li N, et al. Estimation of genomic characteristics by analyzing k-mer frequency in de novo genome projects. arXiv preprint. arXiv:1308.2012.
- Altschul SF, Madden TL, Schäffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17):3389–402.PubMed CentralView ArticlePubMed
- Pontius JU, Mullikin JC, Smith DR, Lindblad-Toh K, Gnerre S, Clamp M, et al. Initial sequence and comparative analysis of the cat genome. Genome Res. 2007; 17(11):1675–89.PubMed CentralView ArticlePubMed
- Smit AF, Hubley R, Green P. RepeatMasker Open-4.0. http://www.repeatmasker.org.. 2013-2015.
- Jurka J, Kapitonov VV, Pavlicek A, Klonowski P, Kohany O, Walichiewicz J. Repbase Update, a database of eukaryotic repetitive elements. Cytogenet Genome Res. 2005; 110(1–4):462–7.View ArticlePubMed
- Benson G. Tandem repeats finder: a program to analyze DNA sequences. Nucleic Acids Res. 1999; 27(2):573.PubMed CentralView ArticlePubMed
- Fanning TG. Origin and evolution of a major feline satellite DNA. J Mol Biol. 1987; 197(4):627–34.View ArticlePubMed
- Santos S, Chaves R, Guedes-Pinto H. Chromosomal localization of the major satellite DNA family (FA-SAT) in the domestic cat. Cytogenet Genome Res. 2003; 107(1–2):119–22.
- Cunningham F, Amode MR, Barrell D, Beal K, Billis K, Brent S, et al. Ensembl 2015. Nucleic Acids Res. 2015; 43(D1):D662—9.View ArticlePubMed
- Birney E, Clamp M, Durbin R. GeneWise and Genomewise. Genome Res. 2004; 14(5):988–95.PubMed CentralView ArticlePubMed
- Stanke M, Waack S. Gene prediction with a hidden Markov model and a new intron submodel. Bioinformatics. 2003; 19(suppl 2):ii215–25.View ArticlePubMed
- Lowe TM, Eddy SR. tRNAscan-SE: a program for improved detection of transfer RNA genes in genomic sequence. Nucleic Acids Res. 1997; 25(5):955–64.PubMed CentralView ArticlePubMed
- Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004; 32(5):1792–7.PubMed CentralView ArticlePubMed
- Nawrocki EP, Kolbe DL, Eddy SR. Infernal 1.0: inference of RNA alignments. Bioinformatics. 2009; 25(10):1335–7.PubMed CentralView ArticlePubMed
- Griffiths-Jones S, Moxon S, Marshall M, Khanna A, Eddy SR, Bateman A. Rfam: annotating non-coding RNAs in complete genomes. Nucleic Acids Res. 2005; 33(suppl 1):D121—4.PubMed
- Griffiths-Jones S, Grocock RJ, Van Dongen S, Bateman A, Enright AJ. miRBase: microRNA sequences, targets and gene nomenclature. Nucleic Acids Res. 2006; 34(suppl 1):D140—4.PubMed
- Lestrade L, Weber MJ. snoRNA-LBME-db a comprehensive database of human H/ACA and C/D box snoRNAs. Nucleic Acids Res. 2006; 34(suppl 1):D158—62.PubMed
- Li H, Durbin R. Fast and accurate short read alignment with Burrows–Wheeler transform. Bioinformatics. 2009; 25(14):1754–60.PubMed CentralView ArticlePubMed
- Nielsen R, Korneliussen T, Albrechtsen A, Li Y, Wang J. SNP calling, genotype calling, and sample allele frequency estimation from new-generation sequencing data. PLOS ONE. 2012; 7(7):e37558.PubMed CentralView ArticlePubMed
- Browning SR, Browning BL. Rapid and accurate haplotype phasing and missing-data inference for whole-genome association studies by use of localized haplotype clustering. Am J Hum Genet. 2007; 81(5):1084–97.PubMed CentralView ArticlePubMed
- Paten B, Diekhans M, Earl D, John JS, Ma J, Suh B, et al. Cactus graphs for genome comparisons. J Comput Biol. 2011; 18(3):469–81.View ArticlePubMed
- Tesler G. Efficient algorithms for multichromosomal genome rearrangements. J Comput Syst Sci. 2002; 65:587–609.View Article
- Murphy WJ, Larkin DM, Everts-van der Wind A, Bourque G, Tesler G, Auvil L, et al.Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps. Science. 2005; 309(5734):613–7.View ArticlePubMed
- Johnson WE, Eizirik E, Pecon-Slattery J, Murphy WJ, Antunes A, Teeling E, et al. The late Miocene radiation of modern Felidae: a genetic assessment. Science. 2006; 311(5757):73–7.View ArticlePubMed
- Alkan C, Kidd JM, Marques-Bonet T, Aksay G, Antonacci F, Hormozdiari F, et al. Personalized copy number and segmental duplication maps using next-generation sequencing. Nat Genet. 2009; 41(10):1061–7.PubMed CentralView ArticlePubMed
- Li H, Coghlan A, Ruan J, Coin LJ, Heriche JK, Osmotherly L, et al. TreeFam: a curated database of phylogenetic trees of animal gene families. Nucleic Acids Res. 2006; 34(suppl 1):D572—80.PubMed
- De Bie T, Cristianini N, Demuth JP, Hahn MW. CAFE: a computational tool for the study of gene family evolution. Bioinformatics. 2006; 22(10):1269–71.View ArticlePubMed
- Yang Z. PAML: a program package for phylogenetic analysis by maximum likelihood. Comput Appl Biosci. 1997; 13(5):555–6.PubMed
- Yang Z, Nielsen R. Codon-substitution models for detecting molecular adaptation at individual sites along specific lineages. Mol Biol Evol. 2002; 19(6):908–17.View ArticlePubMed
- Guindon S, Black M, Rodrigo A. Control of the false discovery rate applied to the detection of positively selected amino acid sites. Mol Biol Evol. 2006; 23(5):919–26.View ArticlePubMed
- Zhang J, Nielsen R, Yang Z. Evaluation of an improved branch-site likelihood method for detecting positive selection at the molecular level. Mol Biol Evol. 2005; 22(12):2472–9.View ArticlePubMed
- Löytynoja A, Goldman N. An algorithm for progressive multiple alignment of sequences with insertions. Proc Natl Acad Sci USA. 2005; 102(30):10557–62.PubMed CentralView ArticlePubMed
- Fletcher W, Yang Z. The effect of insertions, deletions, and alignment errors on the branch-site test of positive selection. Mol Biol Evol. 2010; 27:2257–67.View ArticlePubMed
- Talavera G, Castresana J. Improvement of phylogenies after removing divergent and ambiguously aligned blocks from protein sequence alignments. Syst Biol. 2007; 56(4):564–77.View ArticlePubMed
- Castresana J. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol. 2000; 17(4):540–52.View ArticlePubMed
- Lechner M, Hernandez-Rosales M, Doerr D, Wieseke N, Thévenin A, Stoye J, et al. Orthology detection combining clustering and synteny for very large datasets. PLOS ONE. 2014; 9(8):e105015.PubMed CentralView ArticlePubMed
- Katoh K, Standley DM. MAFFT multiple sequence alignment software version 7: improvements in performance and usability. Mol Biol Evol. 2013; 30(4):772–80.PubMed CentralView ArticlePubMed
- Adzhubei IA, Schmidt S, Peshkin L, Ramensky VE, Gerasimova A, Bork P, et al. A method and server for predicting damaging missense mutations. Nat Methods. 2010; 7(4):248–9.PubMed CentralView ArticlePubMed
- Bosse M, Megens HJ, Madsen O, Paudel Y, Frantz LA, Schook LB, et al. Regions of homozygosity in the porcine genome: consequence of demography and the recombination landscape. PLoS Genet. 2012; 8(11):e1003100.PubMed CentralView ArticlePubMed
- Xue Y, Prado-Martinez J, Sudmant PH, Narasimhan V, Ayub Q, Szpak M, et al. Mountain gorilla genomes reveal the impact of long-term population decline and inbreeding. Science. 2015; 348(6231):242–5.PubMed CentralView ArticlePubMed
- Li H, Durbin R. Inference of human population history from individual whole-genome sequences. Nature. 2011; 475(7357):493–6.PubMed CentralView ArticlePubMed
- Li H, Handsaker B, Wysoker A, Fennell T, Ruan J, Homer N, et al. The sequence alignment/map format and SAMtools. Bioinformatics. 2009; 25(16):2078–9.PubMed CentralView ArticlePubMed
- Harris RS. Improved pairwise alignment of genomic DNA [Ph.D. thesis]. College of Engineering: The Pennsylvania State University; 2007.
- Hach F, Hormozdiari F, Alkan C, Hormozdiari F, Birol I, Eichler EE, et al.mrsFAST: a cache-oblivious algorithm for short-read mapping. Nat Methods. 2010; 7(8):576–7.PubMed CentralView ArticlePubMed
- Tange O, et al. GNU parallel-the command-line power tool. USENIX Mag. 2011; 36(1):42–7.
- Krzywinski M, Schein J, Birol I, Connors J, Gascoyne R, Horsman D, et al. Circos: an information aesthetic for comparative genomics. Genome Res. 2009; 19(9):1639–45.PubMed CentralView ArticlePubMed
- Quinlan AR, Hall IM. BEDTools: a flexible suite of utilities for comparing genomic features. Bioinformatics. 2010; 26(6):841–2.PubMed CentralView ArticlePubMed
- Danecek P, Auton A, Abecasis G, Albers CA, Banks E, DePristo MA, et al. The variant call format and VCFtools. Bioinformatics. 2011; 27(15):2156–8.PubMed CentralView ArticlePubMed