Fig. 4

Targeting of different geminivirus genomes using a single sgRNA. A single IR-sgRNA was capable of targeting the TYLCV, BCTV, and MeMV genomes. a IR-sgRNA (upper sequence) identical to the TYLCV IR sequence but harboring mismatches with the BCTV and MeMV IR sequences (blue) was used to target all three viral genomes. b–d Alignment of Sanger sequenced reads from the IR-targeted region in the TYLCV, BCTV, and MeMV genomes showing the respective targeted modifications. The wild-type (WT) sequence is shown at the top (red) and the PAM is shown in green