Fig. 3

Specific targeting of different viral genomes. RCRII sgRNAs specific for TYLCV and BCTV sequences target only the TYLCV and BCTV genomes, respectively. a T7EI assays showing specific targeting of the TYLCV or BCTV genomes. b Alignment of Sanger sequenced reads from the TYLCV-targeted RCRII region. c Alignment of Sanger sequenced reads from the BCTV-targeted RCRII region. The various indels are indicated by the numbers to the right of the sequences (−x indicates deletion of x nucleotides; +x indicates insertion of x nucleotides; and X > Y indicates change of nucleotide X to nucleotide Y). Arrows indicate the expected sizes of the cleavage products. WT wild type