Fig. 2
From: Spatial reorganization of telomeres in long-lived quiescent cells
Telomeres hypercluster in the long-lived fraction of stationary phase cells. a Representative image of wild-type strain yAT1684 in SP: telomeres are visualized through Rap1-GFP and bud scars within the cell wall are stained with Calcofluor-white. b Picture of a Percoll gradient separation tube of a wild-type yAT1684 SP culture. HD high density, LD low density. The pie chart represents the distribution of LD (green) and HD (orange) cells within the population. Representative fluorescent images of Rap1 tagged with GFP of the LD and HD cell fractions are shown on the right. Percentages indicate the colony-forming ability of the two fractions measured as percentage of colony-forming units (CFU). c Quantification of the distribution of intensity and number of foci of Rap1-GFP images from experiment shown in (b) with our in-house software. Pie charts represent the percentage of cells with telomere hyperclusters within the population (black)