Fig. 1

Genome-wide decreases in spliceosome assembly at exon–intron junctions following PRPF8 depletion. a Spliceosome iCLIP reveals spliceosomal interactions with pre-mRNA that change following PRPF8 depletion at the 5′ splice site. Spliceosome iCLIP cross-link sites summarized into genome-wide maps around splice junctions reveal distinct peaks associated with individual RNA-binding protein (RBP) occupancy. A large reduction in the amplitude of the cross-link peaks located ~20 nucleotides upstream of the 5′ splice site, and immediately adjacent to the exon–intron junction following PRPF8 depletion is indicated by red arrowheads. b Spliceosome iCLIP reveals spliceosomal interactions with pre-mRNA that change following PRPF8 depletion at the 3′ splice site. A reduction in the amplitude of the cross-link peak located ~50 nucleotides upstream of the 3′ splice site following PRPF8 depletion is indicated by red arrowheads