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Fig. 4 | Genome Biology

Fig. 4

From: Errors in RNA-Seq quantification affect genes of relevance to human disease

Fig. 4

Principle components analysis (PCA) of mouse cancer study. a PCA of tumour (red) and normal (blue) RNA-Seq datasets from each of five cell types. Input data are log(FPKM) values after mapping data using STAR and counting only uniquely mapped reads against known mouse genes (stage 1 analysis) (b) PCA of tumour (red) and normal (blue) RNA-Seq datasets from each of five cell types. Input data are log(FPM) values of reads that cannot be assigned to a single gene but can be uniquely assigned to a multi-map group (MMG). The reads used in (b) are only those reads discarded from (a)

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