Fig. 5

Experimental determination of a proximity threshold for FISH signals based on hiBA-FISH distances. a Distance distribution histograms of minimum Red/Green FISH signal distances per Red allele in the indicated cell lines and experimental conditions. ALK hiBA-ALK probe set, NPM hiBA-NPM1 probe set. One pixel = 320 nm. Bin size = one pixel. The first bin includes distances between zero and less than one pixel. The red dashed line represents a threshold of four pixels (1.28 μm). b Same as (a), but for minimum Red/FarRed FISH signal distances