Fig. 2

CRISPR/Cas9 mediates deletion and inversion of a chromosomal iGFP reporter. a Schematic of mouse cells harboring a chromosomal iGFP reporter. LTR is the long terminal repeat of the MSCV retroviral vector. Arrows denote PCR primers. b Cells were co-transfected with pX330 plasmids inversion sgiGFP.3 and sgiGFP.5 (sgiGFP) or control sgRNAs and imaged 72 h later. c FACS analysis to detect the population of GFP-positive cells. The averaged percentage of GFP⁺ cells is indicated (n = 3). d A PCR reaction detected inversion from genomic DNA. An arrowhead indicates the expected inverted band. e A PCR reaction detected deletion bands (arrowhead) from genomic DNA. The percentage of the deletion band intensity is 31.0 ± 7.4 % (n = 2)