Fig. 8

Expression profiles and genotypes for PM19-A1 and A2 across 12 wheat varieties. RNA-seq data were aligned to the seven wheat PM19 genes. a For PM19-A1 two distinct expression patterns were identified across the varieties tested, one showing high expression (Yitpi-like, blue reads) and the other showing low expression (Westonia-like, green reads). b For PM19-A2 no expression differences were found. Wheat variety names are shown to the left of the panels. For PM19-A1 the presence (plus sign) or absence (minus sign) of the 18-bp promoter deletion is indicated. For PM19-A2 the presence (plus sign) or absence (minus sign) of the 12-bp in-frame deletion is indicated. The approximate position of these two polymorphisms, which showed no recombination in any variety, is indicated with a triangle. Other segregating polymorphisms (black vertical lines) are visible across the panels and not linked to the PM19-A1 expression phenotype. c Summary of the expression results for the seven PM19 genes across all the varieties. The numbers indicate counts per million reads. The differences in PM19-A1 expression between the genotypes with and without the 18-bp promoter deletion were highly significant (T-test P-value = 6.1E-05). No significant differences in the expression were found for the other genes