Table 3 Primers for bisulphite sequencing
Gene | Primer | Tm |
|---|---|---|
SOX9_1 | F: 5′-GGATTGGGGTTTTTTATTTTT-3′ | 59°C |
|  | R: 5′-TTCAATTTTCTTCCCTTTCCT-3′ |  |
SOX9_2 | F: 5′-AGGTTATTAGGGTAGATTGGAGG-3′ | 59°C |
|  | R: 5′5AAATACATATCCCATCACAACC-3′ |  |
Gene | Primer | Tm |
|---|---|---|
SOX9_1 | F: 5′-GGATTGGGGTTTTTTATTTTT-3′ | 59°C |
|  | R: 5′-TTCAATTTTCTTCCCTTTCCT-3′ |  |
SOX9_2 | F: 5′-AGGTTATTAGGGTAGATTGGAGG-3′ | 59°C |
|  | R: 5′5AAATACATATCCCATCACAACC-3′ |  |