Figure 2

SNVs in BAsE-Seq and Deep-Seq libraries. (a-d) SNVs in BAsE-Seq libraries Lib_1:9 and Lib_1:99 were identified as true SNVs (red diamonds) or errors (blue dots) using the 'bulk' approach (a,c) or the 'individual' approach (b,d). The frequency of each SNV (y-axis) is plotted against base position in the consensus sequence (x-axis). Additional information is also provided in Tables 1 and 3. (e,f) SNVs from S7.1 were identified using Deep-Seq and BAsE-Seq. The BAsE-Seq library contained an internal standard that was used to calculate the error-free frequency cutoff for the library; hence, only error-free SNVs are shown in the BAsE-Seq analysis of S7.1. (g) The frequency of SNVs detected in the BAsE-Seq library (y-axis) is plotted against the frequency of SNVs detected in the Deep-Seq library (x-axis). All 68 error-free SNVs identified by BAsE-Seq were also identified by Deep-Seq (Pearson correlation coefficient = 0.94).