Figure 2

Frequency of resistance mutations in serial isolates. (A) Frequency of resistance mutations in sequencing reads from serial isolates. Grey background shading indicates that the isolate was resistant to the given drug on the BD BACTEC 460 platform using standard critical concentrations, with the exception of EMB where the shading indicates resistance at 2 μg/ml on the BD MGIT 960 platform (see main text for details). The FLQ resistant isolates were resistant to both ofloxacin and ciprofloxacin. (B) PCR verification of selected resistance mutations, separated and visualized on agarose gel.