From: GoIFISH: a system for the quantification of single cell heterogeneity from IFISH images
Property | GoIFISH | Columbus | CellProfiler | ImageJ | ImageM | CellTracker |
---|---|---|---|---|---|---|
Cost | Open-Source | Proprietary | Open-Source | Open-Source | Open-Source on request | Open-Source |
Real-Time Update | Yes | Yes | No | No | No | Yes |
Background Intensity Subtraction | Yes | Tuning but no subtraction | Correct Illumination Calculate | Flatten Illumination | No | No |
A-Priori knowledge required (eg. cell size or segmentation methods) | No | No | Yes | No | No | No |
Nuclear Segmentation | Yes | Yes | Yes | Yes | Manual | Yes |
Membrane Segmentation | Yes | Yes | Yes | Yes | Manual | Yes |
Spot Detection | Yes | Yes | Yes | Yes | Yes | No |
Batch Processing | CmdLine Only | Yes | Yes | BatchCommand Plugin | No | No |
Visualise Batch Segmentation Results | Yes | No | Yes | No | No | No |
Manual Editing | Yes | No | No | No | Yes | Yes |
Cell Specific Information | Yes | Yes | Yes | Yes | Yes | Yes |
Summary Report | Yes | Yes | Yes | Yes | Yes | Yes |
Topology or summary Maps | Yes | No | Yes | Topit Plugin | No | No |