Regulating large chromatin domains
© BioMed Central Ltd 2002
Published: 15 October 2002
The thymocyte-specific SATB1 (special AT-rich sequence binding 1) protein binds to base-unpairing regions (BURs) of chromosomal DNA within matrix-attachment regions (MARs) and assembles a SATB1 network structure that can regulate gene expression over relatively large distances. In the October 10 Nature, Yasui et al. describe biochemical analysis of SATB1 within BUR-binding complexes (Nature 2002, 419:641-645). They analysed extracts from the thymi of normal and knockout (SATB1 -/- ) mice and found that components of the NURD, CHRAC and ACF chromatin-remodelling complexes co-purified with SATB1. Immunoprecipitation analysis showed that SATB1 recruits histone deacetylases and remodelling complexes, and represses the IL-2Ralpha (inteleukin-2 receptor alpha gene) locus. Changes in nucleosome positioning in the absence of SATB1 could be observed as much as 8 kilobases away, suggesting that mechanisms of this sort play a general a role in global gene regulation.