Minos in mammals
© BioMed Central Ltd 2001
Published: 26 September 2001
Transposon-based technology for insertional mutagenesis of the genome has been widely used in Drosophila and could be adapted for genomic analysis in mammals. Minos is a mobile element of the Tc1/mariner superfamily isolated from Drosophila hydrei. In the September 25 Proceedings of the National Academy of Sciences, Zagoraiou et al. report the use of Minos transposons in mouse tissues (Proc Natl Acad Sci USA 2001, 98:11474-11478). They generated two transgenic mouse lines: one expressing the Minos transposase gene specifically in lymphocytes (under control of the CD2 promoter) and the other carrying a non-autonomous Minos transposon. They crossed these mice and used PCR analysis to look for evidence of transposon excision. Excision was detected in the spleen and thymus of double transgenic animals. The authors then used FISH (fluorescent in situhybridisation) to confirm transposition to other chromosomes. This work demonstrates that transposon-based technology may be developed for functional analysis of the mouse genome.
- Transposition of cloned P elements into Drosophila germ line chromosomes
- Mobile Minos elements from Drosophila hydei encode a two-exon transposase with similarity to the paired DNA-binding domain.
- Proceedings of the National Academy of Sciences , [http://www.pnas.org]