Figure 5

Effects of sequence characteristics on coverage variability. Distributions of (A) hexamer entropy, (B) GC-content, and (C) rRNA sequence similarity for the 100 transcripts with the highest and lowest coefficients of variation for transcript coverage from the plasmid, no selection, rRNA-depleted, and polyA libraries. Asterisks indicate the significance of a Wilcoxon signed-rank test comparing values for the listed sequence characteristics between each pair of groups from the same sample. *P <0.05; **P <0.01; ***P <0.001.