Figure 1

Endothelial cell migration is synergistically enhanced by hypoxia and the PPARβ/δ agonist. (A) Images acquired for each stimulus at 0 hour and 8 hours. HUVECs were treated with the PPARβ/δ agonist (GW501516 100 nM) and/or hypoxia (1% O₂) for 8 hours. The vertical lines indicate the edge of the scratch and the red colored regions at 8 hours show the cell migratory area. Representative images are shown. (B) Quantification of the area of endothelial cell migration. The area colored in red (Figure 1A) was measured and the size relative to the untreated condition is indicated. Data (mean ± standard deviation) are representative of three independent experiments with similar results. *P < 0.05 compared with the untreated condition.