A simple strategy for heritable chromosomal deletions in zebrafish via the combinatorial action of targeting nucleases

Table 4 Germ-line transmission frequency of cyc and sqt nuclease-induced lesions in zebrafish

Targeting nuclease(s)	Number of F₀ screened	Number of mutant F₀s
sqt5TAL + sqt3TAL	56	6 (whole locus deletions)
sqt5TAL + sqtZFN2	28	2 (TSS deletions)
sqtZFN1	92	1 (4 bp insertion)
cyc5TAL + cyc3TAL	36	10 (9 founders with TSS deletions, and 1 with a non-TSS 151 bp deletion and a deletion + inversion + insertion)

For each founder (F₀), at least 30 F1 embryos for sqt, or 22 F1 embryos for cyc were analyzed.

ISSN: 1474-760X