Table 3 Frequency of cyclopia and mid-line defects in cyc and sqt nuclease-injected embryos
Targeting nuclease(s) | Wild type | Cyclopia and midline defects | Abnormal | Dead | Total (N) |
|---|---|---|---|---|---|
6.25 pg cyc5TAL+cyc3TAL | 176 (77.5%) | 30 (13.2%) | 8 (3.5%) | 13 (5.8%) | 227 |
12.5 pg cyc5TAL+cyc3TAL | 134 (60.1%) | 54 (24.2%) | 17 (7.6%) | 18 (8.1%) | 223 |
25 pg cyc5TAL+cyc3TAL | 11 (16.2%) | 27 (39.7%) | 27 (39.7%) | 3 (4.4%) | 68 |
25 pg sqtZFN2 | 62 (53.0%) | 25 (21.4%) | 18 (15.4%) | 12 (10.2%) | 117 |
50 pg sqtZFN2 | 32 (29.1%) | 26 (23.6%) | 37 (33.6%) | 15 (13.6%) | 110 |
25 pg sqt5TAL | 22 (71.0%) | 3 (9.7%) | 0 (0.00%) | 6 (19.3%) | 31 |
50 pg sqt5TAL | 43 (45.7%) | 15 (16.0%) | 18 (19.1%) | 18 (19.1%) | 94 |
25 pg sqt3TAL | 18 (47.4%) | 0 (0.00%) | 5 (13.1%) | 15 (39.5%) | 38 |
50 pg sqt3TAL | 29 (38.2%) | 12 (15.8%) | 15 (19.7%) | 20 (26.3%) | 76 |
25 pg sqt5TAL+sqtZFN2 | 22 (29.3%) | 17 (22.7%) | 19 (25.3%) | 17 (22.7%) | 75 |
25 pg sqt5TAL+sqt3TAL | 30 (36.1%) | 20 (24.1%) | 15 (18.1%) | 18 (21.7%) | 83 |