Figure 5

Effects of intracellular NAD⁺ and Sir2 activity on the expression of PDC1 during the diauxic shift. (a) Relative NAD⁺/NADH ratios when WT strain was grown in YPD at log or post log phase. (b) PDC1 expression in WT and npt1Δ strains growing in YPD medium. (c) Extracellular glucose concentrations as WT, sir2Δ, hst1Δ, and sum1Δ strains enter the diauxic shift. Cells were grown in YPD. (d) Chromosome mapping showing no significant binding of Sir2, Hst1 and Sum1 across the PDC5 ORF. (e) PDC5 expression in WT and mutant strains during the diauxic shift. (f) Effect of a catalytically inactive Sir2-H364Y mutant protein on PDC1 expression during the diauxic shift. Error bars represent standard deviation.