Figure 4

FOXM1 shows differential binding according to ERα status in breast cancer cells. (a) Overlap of FOXM1 binding events between MCF7 (ER-positive cells) and MDA-MB-231 (ER-negative cells). (b) Examples of genomic regions showing FOXM1 binding in MCF7 and MDA-MB-231 cells compared to ER binding in MCF7 cells, contrasting ER-negative regions to ER-positive regions. (c) CEAS analysis showing genomic distribution of FOXM1 binding events in MCF7 and MDA-MB-231. (d) Motif analysis shown as a wordcloud schematic of the significantly enriched motifs present in FOXM1 bound regions in MDA-MB-231 cells and in binding regions overlapping in MCF7 cells. The text size is inversely proportional to the log P-value. (e) Western blot showing FOXM1 and ERα protein levels in six breast cancer lines. (f) FOXM1 ChIP followed by qPCR was performed in ER-positive breast cancer cell lines (MCF7, T47D and ZR-751), ER-negative breast cancer cell lines (MDA-MB-231 and MDA-MB-453) and the normal breast cell line MCF10A to compare FOXM1 binding at known regions of co-binding with ER (from MCF7 ChIP-seq data) to ER-negative binding regions. Data representative of triplicate experiments ± standard deviation.