Figure 3From: The mouse DXZ4 homolog retains Ctcf binding and proximity to Pls3 despite substantial organizational differences compared to the primate macrosatelliteExpression of spliced Dxz4 and promoter characterization. (a) Schematic map of the Dxz4 region representing 72.95 to 73.01 Mb of the mouse X chromosome (mm9). The map is inverted for simplicity and the distal direction toward Pls3 indicated. Open block arrows represent Dxz4 monomers. A downstream CGI is indicated. Immediately below is a map indicating location and type of repeat elements for the interval: LINE, long interspersed nuclear element; LTR, long terminal repeat; SINE, short interspersed nuclear element. Below that are the maps of two putative alternatively spliced transcripts based on expressed sequence tag evidence. (b) Confirmation of spliced transcripts by RT-PCR. Each of the seven panels is an image of an ethidium bromide-stained agarose gel showing RT-PCR results for PCR between the exons indicated above. To the left of each image is the predicted product size. Samples include water control (W) and RNA incubated with (+RT) and without (-RT) reverse transcriptase. (c) DNA sequence feature map of the 1.3-kb region immediately upstream of Dxz4 exon 1 (green). Repetitive elements are indicated above the corresponding colored boxes. Immediately below are the regions cloned upstream of a promoterless luciferase reporter gene: construct A (Con.A) and construct B (Con.B). (d) Luciferase activity measured in NIH/3T3 cell extracts 72 hours after transfection with the promoterless luciferase vector (pGL4.10) or the same vector containing inserts for construct A or B. Fold activation of luciferase is shown to the left. Data represent the mean and standard deviation of replicate experiments each performed in triplicate.Back to article page