Figure 5

Smed-H2B RNAi induces a peak of progeny cells. (a-h) WMISH of Smed-nb.21.11e (a,c,e,g) and Smed-agat-1 (b,d,f,h) in control(RNAi) (a,b) and Smed-H2B(RNAi) animals 1 (c,d), 3 (e,f) and 5 (g,h) days after RNAi. Only one control(RNAi) time point (5 days) is shown since no differences were detected among them. Increased numbers of Smed-nb.21.11e- (c) and Smed-agat-1-positive cells (d) were detected 1 day after RNAi, with a subsequent decrease in numbers for both markers (e-h). (i) Quantification of the level of expression by qRT-PCR of Smed-nb.21.11e and Smed-agat-1 in Smed-H2B(RNAi) animals 1, 3 and 5 days after RNAi, normalized expression and relative to control(RNAi) samples. An upregulation of Smed-nb.21.11e and Smed-agat-1 transcript expression was detected 1 day after RNAi, with a subsequent decrease in levels for both transcripts. (a-h) Anterior is to the left. Scale bars: 500 μm.