Figure 2

Smed-H2B RNAi induces a very quick and specific NB loss. (a-d) Control(RNAi) (a) and Smed-H2B(RNAi) animals cut 1 (b), 3 (c) and 5 (d) days after RNAi, and monitored every 2 days after transection. Only anterior wounds are shown. Only one control(RNAi) time point (5 days) is shown as no differences were detected among them. All Smed-H2B(RNAi) animals fail to regenerate missing tissues, but the earlier time points (b,c) are able to generate a small blastema (white tissue). (e-s) WMISH of Smedwi1 (e,h,k,n,q), Smed-nb.21.11e (f,i,l,o,r) and Smed-agat-1 (g,j,m,p,s) in control(RNAi) (e-g) and Smed-H2B(RNAi) animals 5 (h-j), 10 (k-m), 15 (n-p) and 20 (q-s) days after RNAi. Only one control(RNAi) time point (20 days) is shown since no differences were detected among them. Smedwi-1-positive cells disappear quickly after Smed-H2B(RNAi) (h,k,n,q). Smed-nb.21.11e-positive cells are still detectable 5 days after Smed-H2B(RNAi) (i) but disappear soon after (l,o,r). Smed-agat-1-positive cells progressively disappear and are restricted to the posterior part of the animal (j,m,p,s). (a-d) Anterior is to the top. (e-s) Anterior is to the left. Scale bars: 500 μm. The lables cat 1, cat 2 and cat 3 refer to the definitions previously used by Esienhoffer et al [24].