Figure 6

SRSF3 and SRSF4 contact exons and introns. (a) SRSF3 and SRSF4 crosslink sites mapped around the 5' and 3' splice sites. The position 0 (dotted line) represents the indicated 5' or 3' splice site; the y-axis represents normalized crosslink sites per 10³ nucleotides. The normalization is based on the length distribution of exons and introns (Figure S5 in Additional file 1). The data were smoothed using a Gaussian window (half-width of the window = 5). (b) SRSF3 and SRSF4 crosslink sites mapped to predicted mouse branch points. The position 0 (dotted line) represents the branch point nucleotide. Smoothing as in (a).