Figure 3From: SpliceGrapher: detecting patterns of alternative splicing from RNA-Seq data in the context of gene models and EST dataAmbiguities in RNA-Seq data. This figure demonstrates ambiguities that arise in RNA-Seq data that make isoform prediction challenging. Because there is read coverage across several introns, SpliceGrapher is not able to determine whether this is a result of a single intron retention event, or several independent events.Back to article page