Table 6 Primers used for capillary sequencing of the 23 SNVs and for genotyping the Isl1 mutation
Gene name | Location | Forward primer | Reverse primer |
|---|---|---|---|
1700001K19Rik | 12:111907080 | CTTCTTCTTCCTCTTAATTCTCTCAGG | TTTAGATCCTTATGATGGTGACTCG |
1700104B16Rik | 8:34841236 | TGTAGCAGACTGGGCTTTGC | CTCCCTCAGTCCCTACAAGC |
Acsl3 | 1:78692680 | CTCTCACCTGTGTGCTCTGG | CCTGATCTGCTAATGTCTGTGG |
Bcl2l14 | 6:134377474 | CTTCATCCTAAACAACCAGAAGTCC | AGTGTGATTAGAGCTAGTCCTCTTCTCC |
Btnl7 | 17:34670007 | AGAGAGTTCCTGGCATGTGG | CAGGCTTAGGACTGGAGACG |
Catsper2 | 2:121223476 | CTGGATGTCTTACACTCACTACACTGC | CTATATGTACAGAGGGACCAGTCTTGG |
Col6a3 | 1:92672331 | CAGGCATAAAAGATGGTGTCTCTAAG | GACCAAACCAACAGCAATTGTAAAC |
Creb3l2 | 6:37284584 | GATGCCCTGAGCAGAGAGG | TGCAGAAAGCCAAACCTAGC |
Gm10859 | 2:5833494 | AATCTCAGTTGAGAGAAAACCTACG | GAGATAGCTCAGTCAGTCAGTCAGG |
Gm11149 | 9:49380322 | GACATTCTCTAAAAGCAGAGACATCC | ACGGACTACAGTCTAAAACATCTAAGC |
Gtf3c2 | 5:31476808 | CCTCAAATCCAGGCAAAGG | CTCGTTGCTGTATCTCTGTGC |
H2-Oa | 17:34229420 | CTAACCTGGACTCTGTTTCTTTTTACC | CTACATTTCCACTGACTCTTTCAGAGC |
Ido1 | 8:25703857 | CCGGTAGTGGATGCTGTAGG | CTCTAAGTGACCTCCGTGAGC |
Isl1 | 13:117098488 | CACTGGGCACTCTAAAGTAAACG | TTCTCCGGATTTGGAGTGG |
Mdc1 | 17:35984844 | CATCTGCAGGACTGCCTAGC | TGGGACTTGACCTCTTCTGC |
Olfr424 | 1:176066876 | GGACAAAGAATAACACAGATTTTCC | GAACAAAGGAATGAAGAAGAGG |
Olfr573-ps1 | 7:110091057-8 | AGAGGAAGTAGTACATAGGCTCATGG | CTACTGAAAGAGTTAACTTAGTGGAGAGG |
Olfr749 | 14:51356853 | AGACAGAATGTTGGCTAGTATGTTAGG | CTAATTATCTAGATCGCCTTTGACTCC |
Rsf1 | 7:104809403-4 | GACACTAAAAGTAGAAAGCAGTCACC | GCTTTTCTAGCTTTACAATGACTGG |
Sap30bp | 11:115825338 | CAACACAGGAAATGGACACG | AACCAACAGGACCCAGAGG |
U1 | 1:172958261 | TAAATACTTACCTGGCAGGAGAGATACC | TTATATTGGTGCACTAGCTTCATGC |