Figure 7

Smed-egr-like1 is rapidly induced during regeneration and wound healing. (a) Schematic overview of the whole-worm sample collection approach for SOLiD expression profiling. (b) Sample collection timeline. Regenerating and intact control samples are indicated as red and green dots, respectively. (c, d) Volcano plots showing the negative decadic logarithm of the adjusted P-value and the observed log₂ fold changes 1 h (c) and 6 h (d) after injury. The expression of smed-egr-like1 (red) and smed-runt-like1 (purple) are indicated. The horizontal grey line indicates an adjusted P-value cutoff of 0.001, vertical grey lines indicate log₂ fold change threshold of -0.7 and 0.7. Significantly up- and downregulated genes are shown in yellow and blue, respectively. (e) Schematic diagram illustrating complete and incomplete cuts, triggering a regeneration or wound healing response, respectively, in the animals. (f) Whole mount in situ hybridization analysis of smed-egr-like1 mRNA in regenerating head, trunk, and tail fragments at 1 h (f) and 6 h (f') as well as a wounded animal at 1 h (f''). Anterior is left. Scale bar is approximately 2 mm. (g) Whole mount in situ hybridization analysis of smed-runt-like1 mRNA in regenerating head, trunk, and tail fragments at 1 h (g) and 6 h (g') as well as a wounded animal at 6 h (g''). Scale and orientation as in (f). Smed-runt-like1 is strongly induced in cells close to blastemas and around incisions at 6 h of regeneration.