Mapping of disease-associated variants in admixed populations
© BioMed Central Ltd. 2011
Published: 30 May 2011
Recent developments in high-throughput genotyping and whole-genome sequencing will enhance the identification of disease loci in admixed populations. We discuss how a more refined estimation of ancestry benefits both admixture mapping and association mapping, making disease loci identification in admixed populations more powerful.
High-throughput genotyping and sequencing will enable refined estimation of ancestry, thus enhancing disease loci identification in admixed populations
A major goal of human genetics is to identify genetic variation causally related to either Mendelian or complex diseases. More broadly, a fundamental goal of genetics is to describe the genetic architecture underlying any trait of interest. Most candidate gene studies, linkage studies, or genome-wide association studies to date have focused on European populations, for which large samples of ancestrally homogeneous individuals from relatively homogeneous environments have been established. For example, approximately 90% of genome-wide association studies have been performed using samples of individuals with European ancestry . However, expanding human genetic studies to include diverse worldwide populations is needed to: (i) identify novel loci absent or not readily identifiable in European populations due to low allele frequencies and the resulting low statistical power; (ii) establish the extent to which findings from studies of European populations generalize or transfer to non-European populations; and (iii) study diseases or traits, such as podoconiosis and human African trypanosomiasis (sleeping sickness), present in non-European populations only [2–4].
In this article, we highlight the special value of admixed populations in disease mapping studies. Admixed populations are not ancestrally homogeneous but rather are populations with ancestry from more than one parental population. Admixed populations that have successfully contributed to the mapping of susceptibility loci include African Americans, who have African and European ancestry, and Latino Americans, who have African, European, and Native American ancestry. These admixed populations afford opportunities for the study of health inequalities or group differences, which can occur when there are differences in traits such as disease susceptibility (for example, a 2.8-fold increase in risk for hypertensive heart disease in African Americans compared with European Americans ) or drug response (for example, differential response between populations with African and European ancestry to peginterferon α-2a or peginterferon α-2b combined with ribavirin, which are used to treat chronic infection with the hepatitis C virus ). Such differences result from a combination of environmental and genetic differences, the latter of which are our focus.
Admixture mapping and association mapping studies in admixed populations are poised to enter a new era as a result of the availability of economical high-throughput genotyping and sequencing. To date, ancestry has been estimated using panels containing <10,000 highly ascertained markers known as ancestry-informative markers (AIMs) . Estimation of ancestry improves with panels of approximately 1,000,000 random markers compared with sparse panels of AIMs, leading to increased statistical power and resolution for admixture mapping. Improved estimation of ancestry at the marker level results in decreased false-positive error rates and increased power for association mapping due to the elimination of confounding by ancestry. Highly resolved estimation of local ancestry can also facilitate detection of natural selection in admixed samples. Improved estimation of ancestry will therefore contribute to mapping of disease loci as well as contribute to understanding demographic and adaptive history.
Population genetics of admixture
After one generation of random mating within the meta-population, an individual has inherited one chromosome from each parental population (Figure 1). Local ancestry is still uniform across all loci for a given individual. After a second generation of random mating and beyond, an individual's genome is a mosaic of chromosomal segments with ancestry switching from segment to segment among the parental populations (Figure 1). An ancestry switch refers to a change in ancestry in the interval between two markers and is the result of recombination during meiosis. In this simple example, the proportion of ancestors from each parental population is equal for every admixed individual.
There are several characteristics of admixed populations that are relevant to disease loci mapping. First, not all individuals in an admixed population necessarily have the same proportion of ancestors from each parental population. Second, all loci do not have to share the same genealogical history. These two characteristics of admixed populations are sources of variance that must be accounted for when estimating local ancestry. Third, at any given locus, allele frequencies can vary between the parental populations. The expected allele frequency in the admixed population is the linear combination of the allele frequencies in the parental populations with weights determined by the sample admixture proportion. Fourth, the admixed population can be more genetically diverse than the parental populations if a locus is not polymorphic with the same alleles in all parental populations. For example, suppose a locus is polymorphic in one parental population and monomorphic in a second parental population. In addition, suppose a second locus is monomorphic in the first parental population and polymorphic in the second parental population. The admixed population is expected to be polymorphic at both loci. Fifth, similar to the way in which allele frequencies at a locus may vary, the patterns of covariance between allele frequencies at linked loci, known as linkage disequilibrium, can also differ. As a result, the distribution of haplotype frequencies in the admixed population can be substantially different from the distributions of haplotype frequencies in the parental populations. These latter three characteristics of admixed populations affect how many markers are needed for association mapping and the fine-mapping of functional variants.
Admixture mapping: from AIMs to random markers
Linkage disequilibrium caused by admixture that extends further than background linkage disequilibrium in the parental populations is the basis of admixture mapping [9, 10]. Admixture mapping became practical in 2004 with the development of statistical methods  and panels of AIMs developed from reference databases  (for a historical overview of progress, see Winkler et al. ). Essentially, admixture mapping is designed to evaluate variation in ancestry. Tests of linkage are based on assessing the relationship between phenotype and local ancestry . The standard case-control design involves a comparison of local ancestry between cases and controls, whereas the case-only design involves a comparison of local ancestry to the average local ancestry [11, 14, 15]. The average local ancestry is synonymous with global ancestry and the individual admixture proportion.
Two major challenges remain in admixture mapping. First, inferring ancestry conditional on two parental populations is generally considered to be solved, but inferring ancestry for admixed populations with three or more parental populations remains challenging, particularly when the number of parental populations is unknown. As an example of three-way admixture, Puerto Rican individuals can have varying proportions of African, European, and Native American ancestry [22, 23]. Compared with a prevalence of 7.8% of asthma among European Americans, the prevalence of asthma among Puerto Ricans is 16.6% . Second, admixture mapping involves testing multiple markers across the genome. To maintain control of the false-positive error rate and provide maximum power, the genome-wide significance level must account for the number of markers tested while accounting for correlation of ancestry between markers. Appropriate genome-wide significance levels for admixture mapping are unclear [25–27]: for African Americans, estimates of the number of tests range from 400  to 31,000 .
Refining association mapping in admixed populations
Association mapping is designed to evaluate differences in genotype frequencies. The major challenge for this approach in admixed populations is the risk of false-positive genotype-phenotype associations due to variation in ancestry . There are several techniques to control for this form of confounding for samples of ancestrally homogeneous individuals, including genomic control, structured association testing, principal components, variance components, and propensity scores [16, 29, 30]. These techniques control for confounding at the level of the individual but none controls for confounding at the level of specific markers such as SNPs .
Controlling for confounding due to admixture in association testing
Genotype and global ancestry
Genotype stratified by local ancestry
One way to control for confounding due to local ancestry in association mapping is to simply include local ancestry as an additive covariate. This parametric approach assumes that the effect of local ancestry is additive, analogous to the additive genetic model for genotypes. Alternatively, a non-parametric approach to control for confounding due to local ancestry is stratified regression. Specifically, when testing for association at a locus, one actually performs separate regressions with the subgroups defined by local ancestry. The separate regressions can then be pooled to generate inverse variance-weighted regression coefficients and standard errors. One can also perform subgroup analyses. For example, differences in effect sizes at a locus can be tested by Welch's t test, and differences in reference allele frequencies can be tested using a test of proportions between subgroups. These tests help to address the issue of heterogeneity of genetic associations across populations. Furthermore, we can jointly test for genotype and ancestry effects at each marker .
Imputation is commonly used in association studies to 'fill in' genotypes for untyped markers by leveraging external data on patterns of linkage disequilibrium . For example, the HapMap  or 1000 Genomes CEU  data provide reference data regarding linkage disequilibrium patterns relevant for association studies comprising samples of similar European ancestry. For admixed samples, each parental population may be represented by a separate reference data set. However, it is not yet clear how best to utilize multiple reference data sets to maximize imputation accuracy . Ancestry-aware imputation can be more accurate than not accounting for local ancestry .
In direct contrast to the situation with admixture mapping, approximately 1 million markers are barely sufficient to saturate background linkage disequilibrium in association mapping in populations of European or East Asian ancestry and insufficient for populations of African ancestry, as they are more genetically diverse . In addition to weaker background linkage disequilibrium in populations of African ancestry compared with those of European ancestry, there are more low-frequency and rare variants in populations of African ancestry . Whole-genome sequencing can contribute substantially to association mapping by eliminating the use of tagging variants that achieve poor coverage in genomic regions of weak linkage disequilibrium and by discovering all genetic variants regardless of frequency. Compared with high-throughput genotyping, sequencing will also expedite finding causal variants (that is, genetic variants directly associated with the phenotype of interest).
Several success stories of disease loci mapping in admixed populations have been reported [7, 39], including studies in African Americans for asthma, cancer, and kidney disease. The two diseases for which fine-mapping of the original admixture signal has proceeded the furthest are prostate cancer and end-stage kidney disease.
An admixture signal on chromosome 8q24 was found for prostate cancer in African Americans . The same locus was detected by linkage analysis in Icelanders . At least three blocks of linkage disequilibrium containing several independently associated variants have been identified within this locus [41–44]. Molecular studies identified enhancer elements for the oncogenic transcription factor MYC that regulate tissue-specific expression patterns, which potentially explain why the locus affects risk for breast and colorectal cancer in addition to prostate cancer [45–51]. One of these enhancers interacts with the MYC promoter through binding of the transcription factor complex β-catenin/TCF7L2 [46, 50]. TCF7L2 is the most strongly associated gene for type 2 diabetes , providing in part a genetic basis for the epidemiological association between these two diseases. Thus, although the original admixture signal was detected for prostate cancer, ongoing follow-up studies indicate that the locus also influences breast cancer, colorectal cancer, and type 2 diabetes.
An admixture signal on chromosome 22q13 was found for focal segmental glomerulosclerosis, a cause of end-stage kidney disease, in African Americans [53, 54]. Originally, the candidate gene underlying this signal was thought to be MYH9, which encodes non-muscle myosin heavy chain 9 and is highly expressed in kidney podocytes [53, 54]. Sequence data from the 1000 Genomes Project  included newly discovered variants in a part of chromosome 22q13 that had poor coverage in the International HapMap Project . Using the more comprehensive sequence data, it now appears that the major effect gene is that encoding apolipoprotein L1, which is in linkage disequilibrium with MYH9 . The protein product apolipoprotein L1 has trypanolytic activity; the gene locus appears to be under balancing selection for protection against sleeping sickness at the cost of increased risk for end-stage kidney disease . Starting from the original admixture signal, sequence data have permitted fine-mapping, with the majority of the signal resolved to two alleles located in the last exon of the gene encoding apolipoprotein L1 .
Admixed populations: how many markers should one use?
In the USA, the two most commonly studied admixed populations are African Americans and Latino Americans. Both of these populations are characterized by less than 25 generations since admixture began as a result of maritime European expansion. For both of these admixed populations, the parental populations genetically differ at the intercontinental level. Population differentiation is often measured by FST, which is defined as the ratio of the observed variance in allele frequencies among populations to the variance expected if the populations were randomly mating. FST at the intercontinental level is generally greater than 0.10 . Other examples of admixed populations with this level of population differentiation include Ashkenazi Jews (who have Eastern European and Middle Eastern ancestry), South African Coloureds (who have Bantu-speaking African, European, Indian, Khoisan, and Southeast Asian ancestry), Australian Aboriginals (who have Aboriginal and European ancestry), and Pacific Islanders (who have European and Polynesian ancestry) .
We estimate that <200,000 random markers (that is, markers not ascertained for ancestry informativeness) are sufficient to saturate the signal of linkage disequilibrium due to admixture in the context of admixture mapping for African Americans. A higher density of markers may enable detection of older admixture, because the number of ancestry switches (and the number of markers required to detect all of those ancestry switches) increases as the number of generations since admixture began increases. As a first-pass approximation, a sample is well powered to detect population structure if 1/FST exceeds the geometric mean of the number of unrelated individuals and the number of independent markers . With a sufficiently large sample and dense markers, it might be possible to detect admixture among parental populations that differ at the intracontinental level, or FST of the order of 0.01 , which is relevant for analysis of Northern Europe versus Southern Europe  and East Asian populations . It also may be possible to detect admixture that occurred more distantly in the past (for example, approximately 100 generations ago), for example, in the Uyghurs in western China (who have European and East Asian ancestry) . At a more ancient level, Tishkoff et al. reported evidence of admixture between Bantu-speaking Africans and Khoisans . At an even more ancient level, the proportion of Neandertal ancestry of Eurasians has been estimated to be between 1% and 4% . Characterization of populations with complex patterns of admixture can contribute substantially to our understanding of population history and can also contribute to understanding complex disease.
Bright prospects for the future
The convergence of high-throughput genotyping or sequencing and new methods to infer local ancestry allows for joint admixture and association analysis. Furthermore, sensitivity to detect admixture afforded by the combination of larger samples, denser markers, and improved inferential methods has increased for ancient admixture events and admixture between more closely related populations. Improved estimation of ancestry will benefit association mapping in admixed populations by eliminating the effects of confounding due to variation in ancestry. High-throughput genotyping and sequencing will enable refined estimation of ancestry, making disease loci identification in admixed populations more powerful.
The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official view of the National Institutes of Health. The Howard University Family Study was supported by National Institutes of Health grants S06GM008016-320107 and S06GM008016-380111. Enrollment was carried out at the Howard University General Clinical Research Center, supported by National Institutes of Health grant 2M01RR010284. This research was supported in part by the Intramural Research Program of the Center for Research on Genomics and Global Health. The Center for Research on Genomics and Global Health is supported by the National Human Genome Research Institute, the National Institute of Diabetes and Digestive and Kidney Diseases, the Center for Information Technology, and the Office of the Director at the National Institutes of Health (1ZIAHG200362-02). Genotyping support was provided by the Coriell Institute for Medical Research. The funding bodies had no role in the design of the study, or collection and analysis of data, or in the decision to publish.
- F ST :
the ratio of the observed variance in allele frequencies among populations to the variance expected if the populations were randomly mating
single nucleotide polymorphism.
- Need AC, Goldstein DB: Next generation disparities in human genomics: concerns and remedies. Trends Genet. 2009, 25: 489-494. 10.1016/j.tig.2009.09.012.PubMedView ArticleGoogle Scholar
- McCarthy MI: Casting a wider net for diabetes susceptibility genes. Nat Genet. 2008, 40: 1039-1040. 10.1038/ng0908-1039.PubMedView ArticleGoogle Scholar
- Rosenberg NA, Huang L, Jewett EM, Szpiech ZA, Jankovic I, Boehnke M: Genome-wide association studies in diverse populations. Nat Rev Genet. 2010, 11: 356-366.PubMedPubMed CentralView ArticleGoogle Scholar
- Rotimi CN, Jorde LB: Ancestry and disease in the age of genomic medicine. N Engl J Med. 2010, 363: 1551-1558. 10.1056/NEJMra0911564.PubMedView ArticleGoogle Scholar
- Davey Smith G, Neaton JD, Wentworth D, Stamler R, Stamler J: Mortality differences between black and white men in the USA: contribution of income and other risk factors among men screened for the MRFIT. Lancet. 1998, 351: 934-939.PubMedView ArticleGoogle Scholar
- Ge D, Fellay J, Thompson AJ, Simon JS, Shianna KV, Urban TJ, Heinzen EL, Qiu P, Bertelsen AH, Muir AJ, Sulkowski M, McHutchison JG, Goldstein DB: Genetic variation in IL28B predicts hepatitis C treatment-induced viral clearance. Nature. 2009, 461: 399-401. 10.1038/nature08309.PubMedView ArticleGoogle Scholar
- Winkler CA, Nelson GW, Smith MW: Admixture mapping comes of age. Annu Rev Genomics Hum Genet. 2010, 11: 65-89. 10.1146/annurev-genom-082509-141523.PubMedView ArticleGoogle Scholar
- McKeigue PM: Prospects for admixture mapping of complex traits. Am J Hum Genet. 2005, 76: 1-7. 10.1086/426949.PubMedPubMed CentralView ArticleGoogle Scholar
- Rife DC: Populations of hybrid origin as source material for the detection of linkage. Am J Hum Genet. 1954, 6: 26-33.PubMedPubMed CentralGoogle Scholar
- Chakraborty R, Weiss KM: Admixture as a tool for finding linked genes and detecting that difference from allelic association between loci. Proc Natl Acad Sci USA. 1988, 85: 9119-9123. 10.1073/pnas.85.23.9119.PubMedPubMed CentralView ArticleGoogle Scholar
- Patterson N, Hattangadi N, Lane B, Lohmueller KE, Hafler DA, Oksenberg JR, Hauser SL, Smith MW, O'Brien SJ, Altshuler D, Daly MJ, Reich D: Methods for high-density admixture mapping of disease genes. Am J Hum Genet. 2004, 74: 979-1000. 10.1086/420871.PubMedPubMed CentralView ArticleGoogle Scholar
- Smith MW, Patterson N, Lautenberger JA, Truelove AL, McDonald GJ, Waliszewska A, Kessing BD, Malasky MJ, Scafe C, Le E, De Jager PL, Mignault AA, Yi Z, De Thé G, Essex M, Sankalé JL, Moore JH, Poku K, Phair JP, Goedert JJ, Vlahov D, Williams SM, Tishkoff SA, Winkler CA, De La Vega FM, Woodage T, Sninsky JJ, Hafler DA, Altshuler D, Gilbert DA, et al: A high-density admixture map for disease gene discovery in African Americans. Am J Hum Genet. 2004, 74: 1001-1013. 10.1086/420856.PubMedPubMed CentralView ArticleGoogle Scholar
- McKeigue PM: Mapping genes that underlie ethnic differences in disease risk: methods for detecting linkage in admixed populations, by conditioning on parental admixture. Am J Hum Genet. 1998, 63: 241-251. 10.1086/301908.PubMedPubMed CentralView ArticleGoogle Scholar
- Hoggart CJ, Shriver MD, Kittles RA, Clayton DG, McKeigue PM: Design and analysis of admixture mapping studies. Am J Hum Genet. 2004, 74: 965-978. 10.1086/420855.PubMedPubMed CentralView ArticleGoogle Scholar
- Montana G, Pritchard JK: Statistical tests for admixture mapping with case-control and cases-only data. Am J Hum Genet. 2004, 75: 771-789. 10.1086/425281.PubMedPubMed CentralView ArticleGoogle Scholar
- Price AL, Zaitlen NA, Reich D, Patterson N: New approaches to population stratification in genome-wide association studies. Nat Rev Genet. 2010, 11: 459-463.PubMedPubMed CentralView ArticleGoogle Scholar
- Adeyemo A, Gerry N, Chen G, Herbert A, Doumatey A, Huang H, Zhou J, Lashley K, Chen Y, Christman M, Rotimi C: A genome-wide association study of hypertension and blood pressure in African Americans. PLoS Genet. 2009, 5: e1000564-10.1371/journal.pgen.1000564.PubMedPubMed CentralView ArticleGoogle Scholar
- Chen G, Shriner D, Zhou J, Doumatey A, Huang H, Gerry NP, Herbert A, Christman MF, Chen Y, Dunston GM, Faruque MU, Rotimi CN, Adeyemo A: Development of admixture mapping panels for African Americans from commercial high-density SNP arrays. BMC Genomics. 2010, 11: 417-10.1186/1471-2164-11-417.PubMedPubMed CentralView ArticleGoogle Scholar
- The International HapMap Consortium: A second generation human haplotype map of over 3.1 million SNPs. Nature. 2007, 449: 851-861. 10.1038/nature06258.PubMed CentralView ArticleGoogle Scholar
- Rieseberg LH, Whitton J, Gardner K: Hybrid zones and the genetic architecture of a barrier to gene flow between two sunflower species. Genetics. 1999, 152: 713-727.PubMedPubMed CentralGoogle Scholar
- Price AL, Tandon A, Patterson N, Barnes KC, Rafaels N, Ruczinski I, Beaty TH, Mathias R, Reich D, Myers S: Sensitive detection of chromosomal segments of distinct ancestry in admixed populations. PLoS Genet. 2009, 5: e1000519-10.1371/journal.pgen.1000519.PubMedPubMed CentralView ArticleGoogle Scholar
- Bryc K, Velez C, Karafet T, Moreno-Estrada A, Reynolds A, Auton A, Hammer M, Bustamante CD, Ostrer H: Genome-wide patterns of population structure and admixture among Hispanic/Latino populations. Proc Natl Acad Sci USA. 2010, 107 (Suppl 2): 8954-8961.PubMedPubMed CentralView ArticleGoogle Scholar
- Via M, Gignoux CR, Roth LA, Fejerman L, Galanter J, Choudhry S, Toro-Labrador G, Viera-Vera J, Oleksyk TK, Beckman K, Ziv E, Risch N, Burchard EG, Martínez-Cruzado JC: History shaped the geographic distribution of genomic admixture on the island of Puerto Rico. PLoS ONE. 2011, 6: e16513-10.1371/journal.pone.0016513.PubMedPubMed CentralView ArticleGoogle Scholar
- Akinbami LJ, Moorman JE, Liu X: Asthma prevalence, health care use, and mortality: United States, 2005-2009. Natl Health Stat Report. 2011, 12: 1-14.Google Scholar
- Sha Q, Zhang X, Zhu X, Zhang S: Analytical correction for multiple testing in admixture mapping. Hum Hered. 2006, 62: 55-63. 10.1159/000096094.PubMedView ArticleGoogle Scholar
- Zhu X, Zhang S, Tang H, Cooper R: A classical likelihood based approach for admixture mapping using EM algorithm. Hum Genet. 2006, 120: 431-445. 10.1007/s00439-006-0224-z.PubMedView ArticleGoogle Scholar
- Nalls MA, Wilson JG, Patterson NJ, Tandon A, Zmuda JM, Huntsman S, Garcia M, Hu D, Li R, Beamer BA, Patel KV, Akylbekova EL, Files JC, Hardy CL, Buxbaum SG, Taylor HA, Reich D, Harris TB, Ziv E: Admixture mapping of white cell count: genetic locus responsible for lower white blood cell count in the Health ABC and Jackson Heart Studies. Am J Hum Genet. 2008, 82: 81-87. 10.1016/j.ajhg.2007.09.003.PubMedPubMed CentralView ArticleGoogle Scholar
- Rosenberg NA, Nordborg M: A general population-genetic model for the production by population structure of spurious genotype-phenotype associations in discrete, admixed or spatially distributed populations. Genetics. 2006, 173: 1665-1678. 10.1534/genetics.105.055335.PubMedPubMed CentralView ArticleGoogle Scholar
- Epstein MP, Allen AS, Satten GA: A simple and improved correction for population stratification in case-control studies. Am J Hum Genet. 2007, 80: 921-930. 10.1086/516842.PubMedPubMed CentralView ArticleGoogle Scholar
- Tiwari HK, Barnholtz-Sloan J, Wineinger N, Padilla MA, Vaughan LK, Allison DB: Review and evaluation of methods correcting for population stratification with a focus on underlying statistical principles. Hum Hered. 2008, 66: 67-86. 10.1159/000119107.PubMedPubMed CentralView ArticleGoogle Scholar
- McClellan J, King MC: Why it is time to sequence. Cell. 2010, 142: 353-355. 10.1016/j.cell.2010.07.027.View ArticleGoogle Scholar
- Redden DT, Divers J, Vaughan LK, Tiwari HK, Beasley TM, Fernández JR, Kimberly RP, Feng R, Padilla MA, Liu N, Miller MB, Allison DB: Regional admixture mapping and structured association testing: conceptual unification and an extensible general linear model. PLoS Genet. 2006, 2: e137-10.1371/journal.pgen.0020137.PubMedPubMed CentralView ArticleGoogle Scholar
- Qin H, Morris N, Kang SJ, Li M, Tayo B, Lyon H, Hirschhorn J, Cooper RS, Zhu X: Interrogating local population structure for fine mapping in genome-wide association studies. Bioinformatics. 2010, 26: 2961-2968. 10.1093/bioinformatics/btq560.PubMedPubMed CentralView ArticleGoogle Scholar
- Tang H, Siegmund DO, Johnson NA, Romieu I, London SJ: Joint testing of genotype and ancestry association in admixed families. Genetic Epidemiol. 2010, 34: 783-791. 10.1002/gepi.20520.View ArticleGoogle Scholar
- Marchini J, Howie B: Genotype imputation for genome-wide association studies. Nat Rev Genet. 2010, 11: 499-511. 10.1038/nrg2796.PubMedView ArticleGoogle Scholar
- The 1000 Genomes Project Consortium: A map of human genome variation from population-scale sequencing. Nature. 2010, 467: 1061-1073. 10.1038/nature09534.PubMed CentralView ArticleGoogle Scholar
- Paşaniuc B, Avinery R, Gur T, Skibola CF, Bracci PM, Halperin E: A generic coalescent-based framework for the selection of a reference panel for imputation. Genet Epidemiol. 2010, 34: 773-782. 10.1002/gepi.20505.PubMedView ArticleGoogle Scholar
- Paşaniuc B, Sankararaman S, Kimmel G, Halperin E: Inference of locus-specific ancestry in closely related populations. Bioinformatics. 2009, 25: i213-i221. 10.1093/bioinformatics/btp197.PubMedPubMed CentralView ArticleGoogle Scholar
- Hindorff LA, Sethupathy P, Junkins HA, Ramos EM, Mehta JP, Collins FS, Manolio TA: Potential etiologic and functional implications of genome-wide association loci for human diseases and traits. Proc Natl Acad Sci USA. 2009, 106: 9362-9367. 10.1073/pnas.0903103106.PubMedPubMed CentralView ArticleGoogle Scholar
- Freedman ML, Haiman CA, Patterson N, McDonald GJ, Tandon A, Waliszewska A, Penney K, Steen RG, Ardlie K, John EM, Oakley-Girvan I, Whittemore AS, Cooney KA, Ingles SA, Altshuler D, Henderson BE, Reich D: Admixture mapping identifies 8q24 as a prostate cancer risk locus in African-American men. Proc Natl Acad Sci USA. 2006, 103: 14068-14073. 10.1073/pnas.0605832103.PubMedPubMed CentralView ArticleGoogle Scholar
- Amundadottir LT, Sulem P, Gudmundsson J, Helgason A, Baker A, Agnarsson BA, Sigurdsson A, Benediktsdottir KR, Cazier JB, Sainz J, Jakobsdottir M, Kostic J, Magnusdottir DN, Ghosh S, Agnarsson K, Birgisdottir B, Le Roux L, Olafsdottir A, Blondal T, Andresdottir M, Gretarsdottir OS, Bergthorsson JT, Gudbjartsson D, Gylfason A, Thorleifsson G, Manolescu A, Kristjansson K, Geirsson G, Isaksson H, Douglas J, et al: A common variant associated with prostate cancer in European and African populations. Nat Genet. 2006, 38: 652-658. 10.1038/ng1808.PubMedView ArticleGoogle Scholar
- Gudmundsson J, Sulem P, Manolescu A, Amundadottir LT, Gudbjartsson D, Helgason A, Rafnar T, Bergthorsson JT, Agnarsson BA, Baker A, Sigurdsson A, Benediktsdottir KR, Jakobsdottir M, Xu J, Blondal T, Kostic J, Sun J, Ghosh S, Stacey SN, Mouy M, Saemundsdottir J, Backman VM, Kristjansson K, Tres A, Partin AW, Albers-Akkers MT, Godino-Ivan Marcos J, Walsh PC, Swinkels DW, Navarrete S, et al: Genome-wide association study identifies a second prostate cancer susceptibility variant at 8q24. Nat Genet. 2007, 39: 631-637. 10.1038/ng1999.PubMedView ArticleGoogle Scholar
- Haiman CA, Patterson N, Freedman ML, Myers SR, Pike MC, Waliszewska A, Neubauer J, Tandon A, Schirmer C, McDonald GJ, Greenway SC, Stram DO, Le Marchand L, Kolonel LN, Frasco M, Wong D, Pooler LC, Ardlie K, Oakley-Girvan I, Whittemore AS, Cooney KA, John EM, Ingles SA, Altshuler D, Henderson BE, Reich D: Multiple regions within 8q24 independently affect risk for prostate cancer. Nat Genet. 2007, 39: 638-644. 10.1038/ng2015.PubMedPubMed CentralView ArticleGoogle Scholar
- Yeager M, Orr N, Hayes RB, Jacobs KB, Kraft P, Wacholder S, Minichiello MJ, Fearnhead P, Yu K, Chatterjee N, Wang Z, Welch R, Staats BJ, Calle EE, Feigelson HS, Thun MJ, Rodriguez C, Albanes D, Virtamo J, Weinstein S, Schumacher FR, Giovannucci E, Willett WC, Cancel-Tassin G, Cussenot O, Valeri A, Andriole GL, Gelmann EP, Tucker M, Gerhard DS, et al: Genome-wide association study of prostate cancer identifies a second risk locus at 8q24. Nat Genet. 2007, 39: 645-649. 10.1038/ng2022.PubMedView ArticleGoogle Scholar
- Haiman CA, Le Marchand L, Yamamato J, Stram DO, Sheng X, Kolonel LN, Wu AH, Reich D, Henderson BE: A common genetic risk factor for colorectal and prostate cancer. Nat Genet. 2007, 39: 954-956. 10.1038/ng2098.PubMedPubMed CentralView ArticleGoogle Scholar
- Pomerantz MM, Ahmadiyeh N, Jia L, Herman P, Verzi MP, Doddapaneni H, Beckwith CA, Chan JA, Hills A, Davis M, Yao K, Kehoe SM, Lenz HJ, Haiman CA, Yan C, Henderson BE, Frenkel B, Barretina J, Bass A, Tabernero J, Baselga J, Regan MM, Manak JR, Shivdasani R, Coetzee GA, Freedman ML: The 8q24 cancer risk variant rs6983267 shows long-range interaction with MYC in colorectal cancer. Nat Genet. 2009, 41: 882-884. 10.1038/ng.403.PubMedPubMed CentralView ArticleGoogle Scholar
- Jia L, Landan G, Pomerantz M, Jaschek R, Herman P, Reich D, Yan C, Khalid O, Kantoff P, Oh W, Manak JR, Berman BP, Henderson BE, Frenkel B, Haiman CA, Freedman M, Tanay A, Coetzee GA: Functional enhancers at the gene-poor 8q24 cancer-linked locus. PLoS Genet. 2009, 5: e1000597-10.1371/journal.pgen.1000597.PubMedPubMed CentralView ArticleGoogle Scholar
- Wasserman NF, Aneas I, Nobrega MA: An 8q24 gene desert variant associated with prostate cancer risk confers differential in vivo activity to a MYC enhancer. Genome Res. 2010, 20: 1191-1197. 10.1101/gr.105361.110.PubMedPubMed CentralView ArticleGoogle Scholar
- Ahmadiyeh N, Pomerantz MM, Grisanzio C, Herman P, Jia L, Almendro V, He HH, Brown M, Liu XS, Davis M, Caswell JL, Beckwith CA, Hills A, MacConaill L, Coetzee GA, Regan MM, Freedman ML: 8q24 prostate, breast, and colon cancer risk loci show tissue-specific long-range interaction with MYC. Proc Natl Acad Sci USA. 2010, 107: 9742-9746. 10.1073/pnas.0910668107.PubMedPubMed CentralView ArticleGoogle Scholar
- Sotelo J, Esposito D, Duhagon MA, Banfield K, Mehalko J, Liao H, Stephens RM, Harris TJ, Munroe DJ, Wu X: Long-range enhancers on 8q24 regulate c-Myc. Proc Natl Acad Sci USA. 2010, 107: 3001-3005. 10.1073/pnas.0906067107.PubMedPubMed CentralView ArticleGoogle Scholar
- Wright JB, Brown SJ, Cole MD: Upregulation of c-MYC in cis through a large chromatin loop linked to a cancer risk-associated single-nucleotide polymorphism in colorectal cancer cells. Mol Cell Biol. 2010, 30: 1411-1420. 10.1128/MCB.01384-09.PubMedPubMed CentralView ArticleGoogle Scholar
- Voight BF, Scott LJ, Steinthorsdottir V, Morris AP, Dina C, Welch RP, Zeggini E, Huth C, Aulchenko YS, Thorleifsson G, McCulloch LJ, Ferreira T, Grallert H, Amin N, Wu G, Willer CJ, Raychaudhuri S, McCarroll SA, Langenberg C, Hofmann OM, Dupuis J, Qi L, Segrè AV, van Hoek M, Navarro P, Ardlie K, Balkau B, Benediktsson R, Bennett AJ, Blagieva R, et al: Twelve type 2 diabetes susceptibility loci identified through large-scale association analysis. Nat Genet. 2010, 42: 579-589. 10.1038/ng.609.PubMedPubMed CentralView ArticleGoogle Scholar
- Kopp JB, Smith MW, Nelson GW, Johnson RC, Freedman BI, Bowden DW, Oleksyk T, McKenzie LM, Kajiyama H, Ahuja TS, Berns JS, Briggs W, Cho ME, Dart RA, Kimmel PL, Korbet SM, Michel DM, Mokrzycki MH, Schelling JR, Simon E, Trachtman H, Vlahov D, Winkler CA: MYH9 is a major-effect risk gene for focal segmental glomerulosclerosis. Nat Genet. 2008, 40: 1175-1184. 10.1038/ng.226.PubMedPubMed CentralView ArticleGoogle Scholar
- Kao WHL, Klag MJ, Meoni LA, Reich D, Berthier-Schaad Y, Li M, Coresh J, Patterson N, Tandon A, Powe NR, Fink NE, Sadler JH, Weir MR, Abboud HE, Adler SG, Divers J, Iyengar SK, Freedman BI, Kimmel PL, Knowler WC, Kohn OF, Kramp K, Leehey DJ, Nicholas SB, Pahl MV, Schelling JR, Sedor JR, Thornley-Brown D, Winkler CA, Smith MW, et al: MYH9 is associated with nondiabetic end-stage renal disease in African Americans. Nat Genet. 2008, 40: 1185-1192. 10.1038/ng.232.PubMedView ArticleGoogle Scholar
- The International HapMap 3 Consortium: Integrating common and rare genetic variation in diverse human populations. Nature. 2010, 467: 52-58. 10.1038/nature09298.PubMed CentralView ArticleGoogle Scholar
- Genovese G, Friedman DJ, Ross MD, Lecordier L, Uzureau P, Freedman BI, Bowden DW, Langefeld CD, Oleksyk TK, Uscinski Knob AL, Bernhardy AJ, Hicks PJ, Nelson GW, Vanhollebeke B, Winkler CA, Kopp JB, Pays E, Pollak MR: Association of trypanolytic ApoL1 variants with kidney disease in African Americans. Science. 2010, 329: 841-845. 10.1126/science.1193032.PubMedPubMed CentralView ArticleGoogle Scholar
- Patterson N, Price AL, Reich D: Population structure and eigenanalysis. PLoS Genet. 2006, 2: e190-10.1371/journal.pgen.0020190.PubMedPubMed CentralView ArticleGoogle Scholar
- Tian C, Kosoy R, Lee A, Ransom M, Belmont JW, Gregersen PK, Seldin MF: Analysis of East Asia genetic substructure using genome-wide SNP arrays. PLoS ONE. 2008, 3: e3862-10.1371/journal.pone.0003862.PubMedPubMed CentralView ArticleGoogle Scholar
- Xu S, Jin L: A genome-wide analysis of admixture in Uyghurs and a high-density admixture map for disease-gene discovery. Am J Hum Genet. 2008, 83: 322-336. 10.1016/j.ajhg.2008.08.001.PubMedPubMed CentralView ArticleGoogle Scholar
- Tishkoff SA, Reed FA, Friedlaender FR, Ehret C, Ranciaro A, Froment A, Hirbo JB, Awomoyi AA, Bodo JM, Doumbo O, Ibrahim M, Juma AT, Kotze MJ, Lema G, Moore JH, Mortensen H, Nyambo TB, Omar SA, Powell K, Pretorius GS, Smith MW, Thera MA, Wambebe C, Weber JL, Williams SM: The genetic structure and history of Africans and African Americans. Science. 2009, 324: 1035-1044. 10.1126/science.1172257.PubMedPubMed CentralView ArticleGoogle Scholar
- Green RE, Krause J, Briggs AW, Maricic T, Stenzel U, Kircher M, Patterson N, Li H, Zhai W, Fritz MH-Y, Hansen NF, Durand EY, Malaspinas AS, Jensen JD, Marques-Bonet T, Alkan C, Prüfer K, Meyer M, Burbano HA, Good JM, Schultz R, Aximu-Petri A, Butthof A, Höber B, Höffner B, Siegemund M, Weihmann A, Nusbaum C, Lander ES, Russ C, et al: A draft sequence of the Neandertal genome. Science. 2010, 328: 710-722. 10.1126/science.1188021.PubMedView ArticleGoogle Scholar