Figure 8
From: Identification of novel exons and transcribed regions by chimpanzee transcriptome sequencing
Example of a novel 3' UTR in the UROS gene. (a) The structure of the UROS gene from the UCSC Genome Browser. At the top is a schematic view of the RefSeq gene with exons as black boxes and introns as connective lines. Below are two tracks with predicted splicing events, then follows a set of tracks with detected TRs in the two tissues and at the bottom is a representation of the primers and the experimentally validated junctions between TRs. (b) The experimental validation with RT-PCR. The two TRs representing the novel 3' UTR are on the first two gels and a positive control of the main transcript is on the third gel. The first lane on the gels is 100 bp ladder (molecular weight marker, MWM), then follows the brain sample with (+RT) and without (-RT) reverse transcriptase, followed by the same set of experiments in the liver sample.