Figure 1

Budding index and DNA content. Relevant genotypes of strains are shown. Strains were also deleted for BCK2, and contained plasmid pRS313{P _MET3 ·CLN2}. Except for control strain cln3Δ, all strains also had the endogenous CLN3 gene expressed under the GAL1 promoter. Asynchronous (Asyn) cultures of the indicated strains were grown in raffinose medium lacking methionine; they were thus kept alive by constitutive expression of CLN2. Cells were arrested in G1 (Arrest) by addition of methionine. After most cells were blocked in G1, galactose was added to induce CLN3. Samples were taken every 15 minutes for (a) budding and (b) DNA content evaluation (not all time points are shown). Only one experiment is shown. Somewhat less synchronous but otherwise similar profiles were obtained in a duplicate experiment (data not shown). WT, wild type.