Figure 6

H3K27me3 widely decorates LEM-2 subdomains except at active genes. (a) A representative genomic region showing ChIP-chip signals for LEM-2, H3K9me2, H3K9me3 and H3K27me3. The top panel indicates the chromosomal position of the enlarged region. The y-axes represent MA2C scores (-2 to 2) for LEM-2 ChIP-chip or z-scores (-2 to 2) for LEM-2 ChIP-seq and histone modification ChIP-chip. (b) Average H3K9 and H3K27 methylation profiles at LEM-2 subdomain boundaries. Sliding window averages (100-bp window; 50-bp offset) of ChIP-chip z-scores for indicated histone modifications (blue) or control H3 (gray) are plotted. For comparison, sliding window averages of LEM-2 ChIP-chip MA2C scores (red) are also shown. (c) H3K9 and H3K27 methylation profiles of genes in LEM-2 subdomains or gaps. Top 20% highly expressed (Top 20% expr) or bottom 20% lowly expressed (Bot 20% expr) genes in mixed-stage embryos across the genome are separately plotted. Lines indicate sliding window averages (100-bp window; 50-bp offset) of ChIP-chip z-scores with vertical bars for 95% confidence intervals. TSS, transcript start site; TES, transcript end site.