© BioMed Central Ltd 2009
Published: 26 August 2009
The claudin multigene family encodes tetraspan membrane proteins that are crucial structural and functional components of tight junctions, which have important roles in regulating paracellular permeability and maintaining cell polarity in epithelial and endothelial cell sheets. In mammals, the claudin family consists of 24 members, which exhibit complex tissue-specific patterns of expression. The extracellular loops of claudins from adjacent cells interact with each other to seal the cellular sheet and regulate paracellular transport between the luminal and basolateral spaces. The claudins interact with multiple proteins and are intimately involved in signal transduction to and from the tight junction. Several claudin mouse knockout models have been generated and the diversity of phenotypes observed clearly demonstrates their important roles in the maintenance of tissue integrity in various organs. In addition, mutation of some claudin genes has been causatively associated with human diseases and claudin genes have been found to be deregulated in various cancers. The mechanisms of claudin regulation and their exact roles in normal physiology and disease are being elucidated, but much work remains to be done. The next several years are likely to witness an explosion in our understanding of these proteins, which may, in turn, provide new approaches for the targeted therapy of various diseases.
Gene organization and evolutionary history
In metazoans, biological compartments of different compositions are separated by epithelial (or endothelial) sheets. The transport between these compartments, especially the movement of molecules that can occur in between the cells that make up the cellular sheets (paracellular diffusion), is highly regulated. In vertebrates, the tight junctions (TJs) are the structures responsible for forming the seal that controls paracellular transport. TJs are composed of multiple components, but the tetraspan integral membrane proteins known as claudins are essential for TJ formation and function .
Gene IDs for claudin genes in commonly studied mammals
Human claudin genes and transcript information
Two variants: alternative splicing, coding unaffected
Two variants: alternative transcription start site, different amino termini
Two variants: alternative splicing, coding unaffected
Two variants: alternative transcription start site, different amino termini
Two variants: alternative splicing, different carboxyl termini
Characteristic structural features
The region that shows the most sequence and size heterogeneity among the claudin proteins is the carboxy-terminal tail. It contains a PDZ-domain-binding motif that allows claudins to interact directly with cytoplasmic scaffold ing proteins, such as the TJ-associated proteins MUPP1 , PATJ , ZO-1, ZO-2 and ZO-3, and MAGUKs . Furthermore, the carboxy-terminal tail upstream of the PDZ-binding motif is required to target the protein to the TJ complex  and also functions as a determinant of protein stability and function . The carboxy-terminal tail is the target of various post-translational modifications, such as serine/threonine and tyrosine phosphorylation  and palmitoylation , that can significantly alter claudin localization and function. Most cell types express multiple claudins, and the homotypic and heterotypic interactions of claudins from neighboring cells allow strand pairing and account for the TJ properties , although it appears that heterotypic head-to-head interactions between claudins belonging to two different membranes are limited to certain combinations of claudins .
Localization and function
Claudin proteins were first purified as components of TJs  and are now known to be essential components of TJ structure and function. TJs are found at the most apical part of the lateral surface of a sheet of epithelial cells and serve as a continuous paracellular seal between the apical and basolateral sections [1, 21]. When observed by freeze-fracture microscopy, TJs can be seen to be composed of complex networks of strands, which can be extremely variable in terms of number and complexity depending on the cell type. Claudins are the major constituents of these strands and from various lines of evidence it has been suggested that claudins may be organized as hexamers within the TJs .
Surprisingly, it has been shown that, under certain conditions, claudin proteins can be localized to the cytoplasm in both normal and neoplastic tissues [6, 23]. This cytoplasmic localization may involve claudin phosphorylation . Although the exact roles of cytoplasmic claudin proteins are unknown, they may be related to vesicle trafficking or cell-matrix interactions .
Studies performed by manipulating claudin levels in vitro have established claudins as being crucial in the regulation of the selectivity of paracellular permeability [8, 9, 25]. Overexpression of various claudins in cell lines affects the epithelial resistance and permeability of different ions, and these changes are dependent on the exact claudins expressed. Site-directed mutagenesis of charged residues has shown that the first extracellular loop has an important role in charge selectivity . For example, substituting a negative charge at residue Lys65 in claudin-4 increases Na+ permeability in Madine-Darby canine kidney II cells . Overall, the data from several studies are consistent with a model in which claudin protein levels and combinations within the TJ have a major role in determining paracellular ion selectivity .
Various mouse models have established the importance of claudins in creating barriers and, in some models, highly specific roles have been demonstrated in particular cell types. For example, the Cldn1 knockout mouse model illustrates the importance of this gene in epidermis TJ function. Claudin-1-deficient mice die soon after birth as a consequence of dehydration from transdermal water loss .
Claudin-11 deficient mice show deafness because of the disappearance of TJs from the basal cells of the stria vascularis (the lateral secretory wall of the cochlear duct) [27, 28]. Similarly, Cldn14 homozygous knockout mice have hearing loss, probably because of impaired ion selectivity in one of the epithelial layers in direct contact with the hair cells (the reticular lamina) . Loss of claudin-19 in a mouse model leads to behavioral deficits, which seem to be due to the disappearance of TJs from Schwann cells, leading to abnormal nerve conduction along peripheral myelinated fibers .
Several human diseases have been shown to be caused by mutations in claudin genes. Mutations in the CLDN1 gene result in progressive scaling of the skin and obstruction of bile ducts, known as neonatal sclerosing cholangitis with ichthyosis . The clinical course can vary markedly, from resolution of symptoms to development of liver failure. Mutations in CLDN16 (also known as paracellin-1) cause a rare magnesium wasting disorder characterized by excessive loss of Mg2+ due to kidney malfunction and known as familial hypomagnesemia with hypercalciurea and nephrocalcinosis (FHHNC) . CLDN16 expression is restricted to certain junctions of the thick ascending loop of Henle in the kidney, where magnesium and calcium are reabsorbed paracellularly. It is hypothesized that the reduction in cation permeability causes a reduction in the intraluminal electrical gradient necessary to drive magnesium back into the blood. Mutations in CLDN19 are associated with a similar phenotype to that seen in patients with CLDN16 mutations . CLDN19 mutations are also associated with a large number of ocular conditions, such as macular colobomata, nystagmus and myopia. CLDN14 is expressed along the endocochlear epithelium and, when mutated, causes nonsyndromic recessive deafness DFNB29 , similar to the phenotype observed in claudin-14-deficient mice . Without being directly affected by known mutations, other claudin proteins have been implicated in human pathologies. Claudin-3 and claudin-4 are known to be surface receptors for the Clostridium perfringens enterotoxin in the gut , and claudin-1, claudin-6, and claudin-9 are co-receptors for hepatitis C virus (HCV) entry [36, 37].
Several claudin proteins have been shown to be abnormally expressed in cancers ; for example, claudin-1 is downregulated in breast and colon cancer [38, 39]. These findings are consistent with the long-known fact that TJs are disassembled during tumorigenesis. However, the expression of claudin-3 and claudin-4 has been found to be highly upregulated in multiple cancers . In cancer, over-expressed claudins may have roles in motility, invasion, and survival .
Claudin function is regulated at multiple levels [16, 41]. Most claudin proteins have potential serine and/or threonine phosphorylation sites in their cytoplasmic carboxy-terminal domains and there are reports suggesting that increased phosphorylation could be associated with changes in barrier function. For example, it has been shown that phosphorylation of claudin-3 and claudin-4 by protein kinase A and C, respectively, results in increased paracellular permeability, possibly because of a mislocalization of claudins [24, 42]. Similarly, lysine deficient protein kinase 4 (WNK4) can phosphorylate multiple claudins and increase paracellular permeability . Overall, several claudins are known to be phosphorylated by kinases . Endocytic recycling of claudin proteins is also a potential mechanism of claudin regulation , and palmitoylation  of these proteins has also been found to influence claudin protein stability. At the transcriptional level, transcription factors such as Snail  and GATA-4  can bind to the promoter regions of various claudin genes and affect their expression. Furthermore, there is evidence to support the concept that claudins are downregulated both transcriptionally and post-transcriptionally by various growth factors and cytokines [16, 47].
We are just beginning to unravel the roles of proteins in TJ formation and function. The large number of claudin proteins and the heterogeneity in their patterns of expression emphasize their crucial roles in the development and maintenance of vertebrate tissues. To add to the complexity, it is now becoming apparent that the claudins are intimately involved in signaling to and from the TJ, providing important cues for cell behavior, such as proliferation and differentiation. These molecular pathways are just emerging and will probably become a major focus of research in the field of claudins and TJs. From a practical point of view, a better understanding of TJ formation and regulation may provide novel avenues for the enhancement of drug delivery and absorption. One promising avenue in cancer research is the possible targeting of tumors overexpressing claudin-3 and -4 with the cytotoxic Clostridium perfringens enterotoxin, which specifically binds these proteins . Similarly, the identification of claudins as receptors for HCV entry suggests these molecules as possible targets for drugs that inhibit HCV infection . In addition to improving our knowledge of the mechanisms important in normal tissue development and maintenance, a better understanding of claudin biology may therefore provide new avenues for targeted therapies of several diseases.
We thank members of our laboratory for helpful comments on the manuscript. This work was supported entirely by the Intramural Research Program of the National Institutes of Health, National Institute on Aging.
- Tsukita S, Furuse M: Pores in the wall: claudins constitute tight junction strands containing aqueous pores. J Cell Biol. 2000, 149: 13-16. 10.1083/jcb.149.1.13.PubMedPubMed CentralView ArticleGoogle Scholar
- Kollmar R, Nakamura SK, Kappler JA, Hudspeth AJ: Expression and phylogeny of claudins in vertebrate primordia. Proc Natl Acad Sci USA. 2001, 98: 10196-10201. 10.1073/pnas.171325898.PubMedPubMed CentralView ArticleGoogle Scholar
- Loh YH, Christoffels A, Brenner S, Hunziker W, Venkatesh B: Extensive expansion of the claudin gene family in the teleost fish, Fugu rubripes. Genome Res. 2004, 14: 1248-1257. 10.1101/gr.2400004.PubMedPubMed CentralView ArticleGoogle Scholar
- Wu VM, Schulte J, Hirschi A, Tepass U, Beitel GJ: Sinuous is a Drosophila claudin required for septate junction organization and epithelial tube size control. J Cell Biol. 2004, 164: 313-323. 10.1083/jcb.200309134.PubMedPubMed CentralView ArticleGoogle Scholar
- Hewitt KJ, Agarwal R, Morin PJ: The claudin gene family: expression in normal and neoplastic tissues. BMC Cancer. 2006, 6: 186-10.1186/1471-2407-6-186.PubMedPubMed CentralView ArticleGoogle Scholar
- Morin PJ: Claudin proteins in human cancer: promising new targets for diagnosis and therapy. Cancer Res. 2005, 65: 9603-9606. 10.1158/0008-5472.CAN-05-2782.PubMedView ArticleGoogle Scholar
- Krause G, Winkler L, Mueller SL, Haseloff RF, Piontek J, Blasig IE: Structure and function of claudins. Biochim Biophys Acta. 2008, 1778: 631-645.PubMedView ArticleGoogle Scholar
- Van Itallie CM, Anderson JM: Claudins and epithelial paracellular transport. Annu Rev Physiol. 2006, 68: 403-429. 10.1146/annurev.physiol.68.040104.131404.PubMedView ArticleGoogle Scholar
- Colegio OR, Van Itallie CM, McCrea HJ, Rahner C, Anderson JM: Claudins create charge-selective channels in the paracellular pathway between epithelial cells. Am J Physiol Cell Physiol. 2002, 283: C142-C147.PubMedView ArticleGoogle Scholar
- Angelow S, Ahlstrom R, Yu AS: Biology of claudins. Am J Physiol Renal Physiol. 2008, 295: F867-F876. 10.1152/ajprenal.90264.2008.PubMedPubMed CentralView ArticleGoogle Scholar
- Piontek J, Winkler L, Wolburg H, Muller SL, Zuleger N, Piehl C, Wiesner B, Krause G, Blasig IE: Formation of tight junction: determinants of homophilic interaction between classic claudins. FASEB J. 2008, 22: 146-158. 10.1096/fj.07-8319com.PubMedView ArticleGoogle Scholar
- Hamazaki Y, Itoh M, Sasaki H, Furuse M, Tsukita S: Multi-PDZ domain protein 1 (MUPP1) is concentrated at tight junctions through its possible interaction with claudin-1 and junctional adhesion molecule. J Biol Chem. 2002, 277: 455-461. 10.1074/jbc.M109005200.PubMedView ArticleGoogle Scholar
- Roh MH, Liu CJ, Laurinec S, Margolis B: The carboxyl terminus of zona occludens-3 binds and recruits a mammalian homologue of discs lost to tight junctions. J Biol Chem. 2002, 277: 27501-27509. 10.1074/jbc.M201177200.PubMedView ArticleGoogle Scholar
- Itoh M, Furuse M, Morita K, Kubota K, Saitou M, Tsukita S: Direct binding of three tight junction-associated MAGUKs, ZO-1, ZO-2, and ZO-3, with the COOH termini of claudins. J Cell Biol. 1999, 147: 1351-1363. 10.1083/jcb.147.6.1351.PubMedPubMed CentralView ArticleGoogle Scholar
- Ruffer C, Gerke V: The C-terminal cytoplasmic tail of claudins 1 and 5 but not its PDZ-binding motif is required for apical localization at epithelial and endothelial tight junctions. Eur J Cell Biol. 2004, 83: 135-144. 10.1078/0171-9335-00366.PubMedView ArticleGoogle Scholar
- Gonzalez-Mariscal L, Tapia R, Chamorro D: Crosstalk of tight junction components with signaling pathways. Biochim Biophys Acta. 2008, 1778: 729-756. 10.1016/j.bbamem.2007.08.018.PubMedView ArticleGoogle Scholar
- Van Itallie CM, Gambling TM, Carson JL, Anderson JM: Palmitoylation of claudins is required for efficient tight-junction localization. J Cell Sci. 2005, 118: 1427-1436. 10.1242/jcs.01735.PubMedView ArticleGoogle Scholar
- Tsukita S, Furuse M, Itoh M: Multifunctional strands in tight junctions. Nat Rev Mol Cell Biol. 2001, 2: 285-293. 10.1038/35067088.PubMedView ArticleGoogle Scholar
- Daugherty BL, Ward C, Smith T, Ritzenthaler JD, Koval M: Regulation of heterotypic claudin compatibility. J Biol Chem. 2007, 282: 30005-30013. 10.1074/jbc.M703547200.PubMedView ArticleGoogle Scholar
- Furuse M, Fujita K, Hiiragi T, Fujimoto K, Tsukita S: Claudin-1 and -2: novel integral membrane proteins localizing at tight junctions with no sequence similarity to occludin. J Cell Biol. 1998, 141: 1539-1550. 10.1083/jcb.141.7.1539.PubMedPubMed CentralView ArticleGoogle Scholar
- Morita K, Furuse M, Fujimoto K, Tsukita S: Claudin multigene family encoding four-transmembrane domain protein components of tight junction strands. Proc Natl Acad Sci USA. 1999, 96: 511-516. 10.1073/pnas.96.2.511.PubMedPubMed CentralView ArticleGoogle Scholar
- Mitic LL, Unger VM, Anderson JM: Expression, solubilization, and biochemical characterization of the tight junction transmembrane protein claudin-4. Protein Sci. 2003, 12: 218-227. 10.1110/ps.0233903.PubMedPubMed CentralView ArticleGoogle Scholar
- Blackman B, Russell T, Nordeen SK, Medina D, Neville MC: Claudin 7 expression and localization in the normal murine mammary gland and murine mammary tumors. Breast Cancer Res. 2005, 7: R248-R255. 10.1186/bcr988.PubMedPubMed CentralView ArticleGoogle Scholar
- D'Souza T, Agarwal R, Morin PJ: Phosphorylation of claudin-3 at threonine 192 by cAMP-dependent protein kinase regulates tight junction barrier function in ovarian cancer cells. J Biol Chem. 2005, 280: 26233-26240. 10.1074/jbc.M502003200.PubMedView ArticleGoogle Scholar
- Hou J, Renigunta A, Konrad M, Gomes AS, Schneeberger EE, Paul DL, Waldegger S, Goodenough DA: Claudin-16 and claudin-19 interact and form a cation-selective tight junction complex. J Clin Invest. 2008, 118: 619-628.PubMedPubMed CentralGoogle Scholar
- Furuse M, Hata M, Furuse K, Yoshida Y, Haratake A, Sugitani Y, Noda T, Kubo A, Tsukita S: Claudin-based tight junctions are crucial for the mammalian epidermal barrier: a lesson from claudin-1-deficient mice. J Cell Biol. 2002, 156: 1099-1111. 10.1083/jcb.200110122.PubMedPubMed CentralView ArticleGoogle Scholar
- Gow A, Davies C, Southwood CM, Frolenkov G, Chrustowski M, Ng L, Yamauchi D, Marcus DC, Kachar B: Deafness in Claudin 11-null mice reveals the critical contribution of basal cell tight junctions to stria vascularis function. J Neurosci. 2004, 24: 7051-7062. 10.1523/JNEUROSCI.1640-04.2004.PubMedPubMed CentralView ArticleGoogle Scholar
- Kitajiri S, Miyamoto T, Mineharu A, Sonoda N, Furuse K, Hata M, Sasaki H, Mori Y, Kubota T, Ito J, Furuse M, Tsukita S: Compartmentalization established by claudin-11-based tight junctions in stria vascularis is required for hearing through generation of endocochlear potential. J Cell Sci. 2004, 117: 5087-5096. 10.1242/jcs.01393.PubMedView ArticleGoogle Scholar
- Ben-Yosef T, Belyantseva IA, Saunders TL, Hughes ED, Kawamoto K, Van Itallie CM, Beyer LA, Halsey K, Gardner DJ, Wilcox ER, Rasmussen J, Anderson JM, Dolan DF, Forge A, Raphael Y, Camper SA, Friedman TB: Claudin 14 knockout mice, a model for autosomal recessive deafness DFNB29, are deaf due to cochlear hair cell degeneration. Hum Mol Genet. 2003, 12: 2049-2061. 10.1093/hmg/ddg210.PubMedView ArticleGoogle Scholar
- Miyamoto T, Morita K, Takemoto D, Takeuchi K, Kitano Y, Miyakawa T, Nakayama K, Okamura Y, Sasaki H, Miyachi Y, Furuse M, Tsukita S: Tight junctions in Schwann cells of peripheral myelinated axons: a lesson from claudin-19-deficient mice. J Cell Biol. 2005, 169: 527-538. 10.1083/jcb.200501154.PubMedPubMed CentralView ArticleGoogle Scholar
- Hadj-Rabia S, Baala L, Vabres P, Hamel-Teillac D, Jacquemin E, Fabre M, Lyonnet S, De Prost Y, Munnich A, Hadchouel M, Smahi A: Claudin-1 gene mutations in neonatal sclerosing cholangitis associated with ichthyosis: a tight junction disease. Gastroenterology. 2004, 127: 1386-1390. 10.1053/j.gastro.2004.07.022.PubMedView ArticleGoogle Scholar
- Simon DB, Lu Y, Choate KA, Velazquez H, Al-Sabban E, Praga M, Casari G, Bettinelli A, Colussi G, Rodriguez-Soriano J, McCredie D, Milford D, Sanjad S, Lifton RP: Paracellin-1, a renal tight junction protein required for paracellular Mg2+ resorption. Science. 1999, 285: 103-106. 10.1126/science.285.5424.103.PubMedView ArticleGoogle Scholar
- Konrad M, Schaller A, Seelow D, Pandey AV, Waldegger S, Lesslauer A, Vitzthum H, Suzuki Y, Luk JM, Becker C, Schlingmann KP, Schmid M, Rodriguez-Soriano J, Ariceta G, Cano F, Enriquez R, Juppner H, Bakkaloglu SA, Hediger MA, Gallati S, Neuhauss SC, Nurnberg P, Weber S: Mutations in the tight-junction gene claudin 19 (CLDN19) are associated with renal magnesium wasting, renal failure, and severe ocular involvement. Am J Hum Genet. 2006, 79: 949-957. 10.1086/508617.PubMedPubMed CentralView ArticleGoogle Scholar
- Wilcox ER, Burton QL, Naz S, Riazuddin S, Smith TN, Ploplis B, Belyantseva I, Ben-Yosef T, Liburd NA, Morell RJ, Kachar B, Wu DK, Griffith AJ, Riazuddin S, Friedman TB: Mutations in the gene encoding tight junction claudin-14 cause autosomal recessive deafness DFNB29. Cell. 2001, 104: 165-172. 10.1016/S0092-8674(01)00200-8.PubMedView ArticleGoogle Scholar
- Katahira J, Sugiyama H, Inoue N, Horiguchi Y, Matsuda M, Sugimoto N: Clostridium perfringens enterotoxin utilizes two structurally related membrane proteins as functional receptors in vivo. J Biol Chem. 1997, 272: 26652-26658. 10.1074/jbc.272.42.26652.PubMedView ArticleGoogle Scholar
- Evans MJ, von Hahn T, Tscherne DM, Syder AJ, Panis M, Wolk B, Hatziioannou T, McKeating JA, Bieniasz PD, Rice CM: Claudin-1 is a hepatitis C virus co-receptor required for a late step in entry. Nature. 2007, 446: 801-805. 10.1038/nature05654.PubMedView ArticleGoogle Scholar
- Zheng A, Yuan F, Li Y, Zhu F, Hou P, Li J, Song X, Ding M, Deng H: Claudin-6 and claudin-9 function as additional coreceptors for hepatitis C virus. J Virol. 2007, 81: 12465-12471. 10.1128/JVI.01457-07.PubMedPubMed CentralView ArticleGoogle Scholar
- Kramer F, White K, Kubbies M, Swisshelm K, Weber BH: Genomic organization of claudin-1 and its assessment in hereditary and sporadic breast cancer. Hum Genet. 2000, 107: 249-256. 10.1007/s004390000375.PubMedView ArticleGoogle Scholar
- Resnick MB, Konkin T, Routhier J, Sabo E, Pricolo VE: Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study. Mod Pathol. 2005, 18: 511-518. 10.1038/modpathol.3800301.PubMedView ArticleGoogle Scholar
- Agarwal R, D'Souza T, Morin PJ: Claudin-3 and claudin-4 expression in ovarian epithelial cells enhances invasion and is associated with increased matrix metalloproteinase-2 activity. Cancer Res. 2005, 65: 7378-7385. 10.1158/0008-5472.CAN-05-1036.PubMedView ArticleGoogle Scholar
- Findley MK, Koval M: Regulation and roles for claudin-family tight junction proteins. IUBMB Life. 2009, 61: 431-437. 10.1002/iub.175.PubMedPubMed CentralView ArticleGoogle Scholar
- D'Souza T, Indig FE, Morin PJ: Phosphorylation of claudin-4 by PKCepsilon regulates tight junction barrier function in ovarian cancer cells. Exp Cell Res. 2007, 313: 3364-3375. 10.1016/j.yexcr.2007.06.026.PubMedPubMed CentralView ArticleGoogle Scholar
- Yamauchi K, Rai T, Kobayashi K, Sohara E, Suzuki T, Itoh T, Suda S, Hayama A, Sasaki S, Uchida S: Disease-causing mutant WNK4 increases paracellular chloride permeability and phosphorylates claudins. Proc Natl Acad Sci USA. 2004, 101: 4690-4694. 10.1073/pnas.0306924101.PubMedPubMed CentralView ArticleGoogle Scholar
- Matsuda M, Kubo A, Furuse M, Tsukita S: A peculiar internalization of claudins, tight junction-specific adhesion molecules, during the intercellular movement of epithelial cells. J Cell Sci. 2004, 117: 1247-1257. 10.1242/jcs.00972.PubMedView ArticleGoogle Scholar
- Ikenouchi J, Matsuda M, Furuse M, Tsukita S: Regulation of tight junctions during the epithelium-mesenchyme transition: direct repression of the gene expression of claudins/occludin by Snail. J Cell Sci. 2003, 116: 1959-1967. 10.1242/jcs.00389.PubMedView ArticleGoogle Scholar
- Escaffit F, Boudreau F, Beaulieu JF: Differential expression of claudin-2 along the human intestine: implication of GATA-4 in the maintenance of claudin-2 in differentiating cells. J Cell Physiol. 2005, 203: 15-26. 10.1002/jcp.20189.PubMedView ArticleGoogle Scholar
- Singh AB, Harris RC: Epidermal growth factor receptor activation differentially regulates claudin expression and enhances transepithelial resistance in Madin-Darby canine kidney cells. J Biol Chem. 2004, 279: 3543-3552. 10.1074/jbc.M308682200.PubMedView ArticleGoogle Scholar