Figure 2

Rpd3p is bound upstream of several target genes after stress. Rpd3-myc binding upstream of several genes (including the positive control INO1, PS gene GAR1, RP gene RPL16A, and iESR genes UBC5 and XKS1) was assessed using ChIP before and 10 minutes after 0.4 mM H₂O₂ treatment or cold phosphate-buffered saline shock (see Materials and methods for details). The log2 enrichment of each fragment recovered from the Rpd3-myc expressing strain versus an untagged control strain is shown, for unstressed cells and cells responding to stress, according to the key on the right. Error bars represent the standard deviation of biological triplicates. The enrichment of each locus in whole-cell extracts (WCE) is shown as a control.