Figure 1

Rpd3p is required for stress-dependent activation of the environmental stress response. Gene expression in wild-type and rpd3Δ cells responding to 25°C to 37°C heat shock (left panels), 0.4 mM H₂O₂ treatment (middle panels), or 0.75 M NaCl exposure (right panels) as described in Materials and methods. (a) The gene expression diagram represents the induced (red) or repressed (green) expression measurements of each gene (represented as rows) in the protein synthesis (PS), ribosomal protein (RP), and induced environmental stress response (iESR) gene groups for each microarray experiment (represented as columns organized temporally within each time course). The difference ('dif.') between wild type and rpd3Δ is represented to the right of each expression diagram: yellow indicates weaker repression and blue indicates weaker induction in the rpd3Δ mutant. Basal expression differences between rpd3Δ and wild type grown in the absence of stress are also shown. (b) The average log₂ expression change of genes in the PS, RP, and iESR subgroups shown in (a) plotted for wild type and rpd3Δ cells. Time points with statistically smaller changes in expression in rpd3Δ cells (P < 0.01, paired t-test) are indicated with an asterisk.