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Table 8 EST clusters without KOG assignment predicted to be highly 2N-specific based on statistical comparison of libraries

From: Transcriptome analysis of functional differentiation between haploid and diploid cells of Emiliania huxleyi, a globally significant photosynthetic calcifying cell

Cluster ID

Number of 2N ESTs

P-value

Homolog ID

Homolog description

BLAST

GS00092

6

7 × 10-17

*B1X317_CYAA5

Putative uncharacterized protein

7 × 10-17

GS03351

14

2 × 10-06

*Q0 MYU5_EMIHU

Putative arachidonate 15-lipoxygenase second type

2 × 10-06

GS05210

7

1 × 10-25

*C1AEM4_GEMAT

Putative glutamine cyclotransferase

1 × 10-25

GS01732

8

1 × 10-19

*A7WPV6_KARMI

Putative uncharacterized protein

1 × 10-19

GS02223

7

1 × 10-31

*C1 MGG4_9CHLO

Predicted protein

1 × 10-31

GS05779

6

2 × 10-17

*C1E2K5_9CHLO

Predicted protein

2 × 10-17

GS06362

6

2 × 10-15

*A9V2G5_MONBE

Predicted protein

2 × 10-15

GS00766

7

3 × 10-07

*B7G9 M0_PHATR

Predicted protein

3 × 10-07

GS03302

7

1 × 10-32

*B7G0S2_PHATR

Predicted protein

1 × 10-32

GS03476

9

2 × 10-09

*B7FQM3_PHATR

Predicted protein

2 × 10-09

GS00513

8

6 × 10-08

*Q7V952_PROMM

Putative uncharacterized protein

6 × 10-08

GS01720

9

8 × 10-06

*B2ZYD9_9CAUD

Nucleoside-diphosphate-sugar pyrophosphorylase-like protein

8 × 10-06

GS05985

7

6 × 10-06

*B0J8I4_RHILT

Putative uncharacterized protein

6 × 10-06

GS01421

6

7 × 10-11

*B9S8J5_RICCO

Putative uncharacterized protein

7 × 10-11

GS05596

8

2 × 10-11

*B8 MI73_TALSN

Putative uncharacterized protein

2 × 10-11

GS00659

6

5 × 10-22

*A4VDD7_TETTH

Putative uncharacterized protein

5 × 10-22

GS11002

16

7.6 × 10-6

   

GS02507

12

1.2 × 10-4

   

GS01164

10

4.9 × 10-4

   

GS01802

10

4.9 × 10-4

   
  1. Only clusters with zero ESTs originating from the 1N library are shown, and only the orphans confirmed by RT-PCR are included in this table. Homolog IDs are marked as in Table 5. Clusters in bold were chosen for RT-PCR validation (cluster GS11002 is shown in bold italics, the only cluster tested in which abundant RT-PCR product could also be detected from 1N cells). Additional data file 5 gives a complete list of all clusters predicted to be 2N-specific by statistical comparison of libraries.