Figure 6
From: Plastic architecture of bacterial genome revealed by comparative genomics of Photorhabdus variants
Duplication of region B. (a) Quantitative PCR was carried out for mrfA (plu0769) and dnaQ (plu0943) using genomic DNA from TT01α/I and VAR* variants and specific internal primers for each gene. pilN (plu1051) and fliC (plu1954) were used for negative controls. PCR was performed in triplicate and data are presented as ratios, with gyrB as the control gene (95% confidence limits). (b, c) Pulsed field gel electrophoresis (PFGE) of NotI-hydrolyzed genomic DNA from TT01α/I and the six variants following by Southern blot and hybridization with a probe covering the region B (probe B). The PFGE conditions allow separation of NotI fragments between 50 and 400 kb (panel b) or between 350 and 1,350 kb (panel c). Gray arrows indicate fragments that hybridize with the probe B. Lane 1: TT01/I. Lane 2: TT01/II. Lane 3: TT01α/I. Lane 4: TT01α/II. Lane 5: TT01α'/II. Lane 6: VAR*. Lane 7: REV.