Figure 3

Example artificial vector sequence mapped to two natural plasmids. The vector sequence is shown in the middle (Phagemid cloning vector pTZ19R), which shares sequence with both the E. coli plasmid pCA4, and the Erwinia amylovora plasmid pEA2.8. Lines connecting the three sequences mark the beginning of exact matches between the artificial sequence and the two respective plasmids. The number next to each line is the length of exact match (for matches of 100 or more bases). Functional annotation for the artificial vector sequence is given above the sequence (RS denotes recombination site). Position 614 marks the starting point of the shortest signature found (k = 23). (Not drawn to scale.)