Figure 7From: Systems biology-defined NF-κB regulons, interacting signal pathways and networks are implicated in the malignant phenotype of head and neck cancer cell lines differing in p53 statusBinding activity and motif logo of RELA and NFκB1. (a) The basal and inducible binding activity of RELA or NFκB1 were tested using TransAM NFκB family kit in UM-SCC 1, 6 and 22B cells after TNF-α (2000 units/ml) treatment. 'Raji' and 'Neg' represent positive and negative controls, respectively. (b) Binding activity of RELA and NFκB1 in the promoter of NF-κB target genes. The promoter sequences with putative RELA or NFκB1 binding sites were synthesized as 50-mer oligos and biotin labeled, and the assays were performed using TransAM flexi NFκB family kit. *P < 0.05 compared with the control (t test). (c) Motif logos of RELA and NFκB1 were generated from 202 and 151 genes differentially expressed in UM-SCC with their putative binding sites, respectively (upper panels). Motif logos of RELA and NFκB1 from Transfac were included for the comparison (lower panels).Back to article page