Figure 5
Spindle defects observed for RNAi phenotypes using confocal fluorescence microscopy. Cells transfected with single (double-stranded) small interfering RNA targeting (a) KIAA1604, (b) KIAA1569/Cep192, (c) FLJ10460/Cep27, (d) SON, (e) KIAA1160, (f) FLJ13111/CENP-T, (g) SMU-1, (h) C18orf24/SKA1, and (i) negative control. Cells were stained for α-tubulin (green), phospho-histone H3 (pHis; blue), and the CREST protein (red). Scale bar represents 5 μm.